Proteomics

Dataset Information

0

LC-MS/MS analysis of isolated murine brain endothelial cells (EC) and full brain tissue (FT) preparations from 3-months-old mice followed by label-free quantification (LFQ)


ABSTRACT: Age-related decline in brain endothelial cell (BEC) function critically contributes to cerebrovascular and neurodegenerative disease. Comprehensive atlases of the BEC transcriptome have become available but results from proteomic profiling are lacking. To gain insights into endothelial pathways affected by aging, we developed a magnetic-activated cell sorting (MACS)-based mouse BEC enrichment protocol compatible with high-resolution mass-spectrometry based proteomics. In this experiment, we have compared MACS sorted BEC and full brain tissue a massive enrichment of endothelial markers including Nos3, Cdh5, and Pecam1 in BECs compared to FT.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain, Endothelial Cell

SUBMITTER: Stephan Mueller  

LAB HEAD: Stefan F. Lichtenthaler

PROVIDER: PXD044993 | Pride | 2024-02-15

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
EC_1_Slot1-28_1_2259.d.zip Other
EC_2_Slot1-29_1_2260.d.zip Other
EC_3_Slot1-30_1_2261.d.zip Other
EC_4_Slot1-31_1_2262.d.zip Other
FullTissue_1_Slot1-36_1_2265.d.zip Other
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