Dataset Information

The role of inverse autotransporter FdeC in Avian Pathogenic Escherichia coli adhesion

ABSTRACT: Avian Pathogenic Escherichia coli (APEC) poses a significant threat to the global poultry industry and has also zoonotic potential, meaning it could infect humans. One critical factor in APEC infection is its ability to adhere to host tissues, a process mediated by a protein called FdeC among others. This protein is present in pathogenic E. coli strains but is often nonfunctional in nonpathogenic ones. Our study focused on understanding the role of FdeC in APEC's ability to bind to chicken intestinal cells. Through an extensive screen of 2000 transposon mutants from the APEC IMT5155 strain, we discovered that a specific mutation in the fdeC gene led to enhanced adhesion. However, a direct deletion of the fdeC gene did not produce the same effect under standard conditions, which led us to investigate the environmental factors that might regulate FdeC expression. We found that FdeC's expression is highly dependent on the environmental conditions such as temperature and pH. Using optimized conditions for FdeC expression, we observed significantly higher adhesion and motility levels in the fdeC-deleted IMT5155 strain compared to the wild type. To understand the mechanisms behind this, we employed mass spectrometry and discovered that the absence of FdeC led to changes in ion transport and downregulated a protein called YbjN, which usually inhibits bacterial movement. Collectively, our findings suggest that FdeC has host-dependent expression and contributes to enhancement of bacterial fitness by modulating motility during colonization.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Escherichia Coli

SUBMITTER: Fernando Garcia

LAB HEAD: Fernando Garcia Martinez

PROVIDER: PXD045075 | Pride | 2024-06-16

REPOSITORIES: Pride

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			Action	DRS
	20211005_ECOLI_37C_FdeC_01.raw	Raw
	20211005_ECOLI_37C_FdeC_02.raw	Raw
	20211005_ECOLI_37C_FdeC_03.raw	Raw
	20211005_ECOLI_37C_FdeC_04.raw	Raw
	20211005_ECOLI_37C_FdeC_05.raw	Raw

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FdeC expression regulates motility and adhesion of the avian pathogenic Escherichia coli strain IMT5155.

Aleksandrowicz Adrianna A Kjærup Rikke Brødsgaard RB Grzymajło Krzysztof K Martinez Fernando Garcia FG Muñoz Javier J Borowska Dominika D Sives Samantha S Vervelde Lonneke L Dalgaard Tina Sørensen TS Kingsley Robert A RA Kolenda Rafał R

Veterinary research 20240531 1

Adaptation of avian pathogenic E. coli (APEC) to changing host environments including virulence factors expression is vital for disease progression. FdeC is an autotransporter adhesin that plays a role in uropathogenic Escherichia coli (UPEC) adhesion to epithelial cells. Expression of fdeC is known to be regulated by environmental conditions in UPEC and Shiga toxin-producing E. coli (STEC). The observation in a previous study that an APEC strain IMT5155 in which the fdeC gene was disrupted by a ...[more]

PMID: 38822378

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