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TGM1 interaction partners identification reveals possible TGM1 posttranslational modifiers

ABSTRACT: TGM1 is an enzyme that cross-links structural proteins in the uppermost granular layer of the epidermis to form cornified envelopes. Cornified envelopes ensure functional stratum corneum and epidermal barrier formation. It has been suggested that the activity of TGM1 is regulated on a posttranslational level. To identify endogenous TGM1 interactors, Virotrap has been performed.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Marta Surbek

LAB HEAD: dr. Leopold

PROVIDER: PXD045105 | Pride | 2024-01-26

REPOSITORIES: Pride

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			Action	DRS
	B102849_Ap_110uPAc-2_CMB-1114_KRGEV_Tessa_Virotrap-eDHFR-1.raw	Raw
	B102850_Ap_110uPAc-2_CMB-1114_KRGEV_Tessa_Virotrap-eDHFR-2.raw	Raw
	B102853_Ap_110uPAc-2_CMB-1114_KRGEV_Tessa_Virotrap-eDHFR-3.raw	Raw
	B102855_Ap_110uPAc-2_CMB-1114_KRGEV_Tessa_Virotrap-eDHFR-4.raw	Raw
	B102857_Ap_110uPAc-2_CMB-1114_KRGEV_Tessa_Virotrap-TGM1-1.raw	Raw

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Publications

Cornification of keratinocytes is associated with differential changes in the catalytic activity and the immunoreactivity of transglutaminase-1.

Surbek Marta M Van de Steene Tessa T Sachslehner Attila Placido AP Golabi Bahar B Griss Johannes J Eyckerman Sven S Gevaert Kris K Eckhart Leopold L

Scientific reports 20231206 1

Transglutaminase 1 (TGM1) plays an essential role in skin barrier formation by cross-linking proteins in differentiated keratinocytes. Here, we established a protocol for the antibody-dependent detection of TGM1 protein and the parallel detection of TGM activity. TGM1 immunoreactivity initially increased and co-localized with membrane-associated TGM activity during keratinocyte differentiation. TGM activity persisted upon further differentiation of keratinocytes, whereas TGM1 immunoreactivity wa ...[more]

PMID: 38057394

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