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Impact of anesthetics on rat hippocampus and neocortex: a comprehensive proteomic study based on label-free mass spectrometry

ABSTRACT: In this work, in vivo experiment simulating a general anesthesia and ICU sedation was designed to assess the impact of two intravenous (midazolam, dexmedetomidine) and two inhalational (isoflurane, desflurane) agents to neuronal centers for cognition (neocortex), learning, and memory (hippocampus). More than 3600 proteins were quantified across both neocortex and hippocampus. Proteomic study revealed relatively mild effects of anesthetics, nevertheless, protein dysregulation uncovered possible different effect of isoflurane (and midazolam) compared to desflurane (and dexmedetomidine) to neocortical and hippocampal proteins.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Marie Vajrychová

LAB HEAD: Marie Vajrychova

PROVIDER: PXD045159 | Pride | 2024-05-23

REPOSITORIES: Pride

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Impact of anesthetics on rat hippocampus and neocortex: A comprehensive proteomic study based on label-free mass spectrometry.

Astapenko David D Vajrychova Marie M Fabrik Ivo I Kupcik Rudolf R Pimkova Kristyna K Tambor Vojtech V Radochova Vera V Cerny Vladimir V

Heliyon 20240305 6

Anesthesia is regarded as an important milestone in medicine. However, the negative effect on memory and learning has been observed. In addition, the impact of anesthetics on postoperative cognitive functions is still discussed. In this work, <i>in vivo</i> experiment simulating a general anesthesia and ICU sedation was designed to assess the impact of two intravenous (midazolam, dexmedetomidine) and two inhalational (isoflurane, desflurane) agents on neuronal centers for cognition (neocortex), ...[more]

PMID: 38509933

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