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DOPA formation on tyrosine Y150 of Ribonucleotide reductase QSK R2e variant from Gardnerella vaginalis.

ABSTRACT: With a crystal structure and mass spectrometry on recombinantly expressed and purified Ribonucleotide reductase QSK R2e variant from Gardnerella vaginalis we show that this protein has a modified DOPA (3,4-Dihydroxy-L-phenylalanine) in the active site analogous to what was observed for the VPK variant. Moreover, we show that this DOPA formation is facilitated by the coexpression of partner proteins NrdI and surprisingly also NrdH and R1 from the same organism.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Gardnerella Vaginalis

SUBMITTER: Rui Branca

LAB HEAD: Janne Lehtio

PROVIDER: PXD045227 | Pride | 2023-09-08

REPOSITORIES: Pride

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			Action	DRS
	Juliane_John_Gerdnerella_R2_2023june_PEAKS11_exports.tgz	Other
	Juliane_R2_Gardnerella_vaginalis_GvNrdF_1A_230523.raw	Raw
	Juliane_R2_Gardnerella_vaginalis_GvNrdF_1A_230529.raw	Raw
	Juliane_R2_Gardnerella_vaginalis_GvNrdF_1A_230601.raw	Raw
	Juliane_R2_Gardnerella_vaginalis_GvNrdF_1B_230529.raw	Raw

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