Dataset Information

Time-course of ischemia-reperfusion in a mouse model

ABSTRACT: We have performed an unbiased, open-search analysis of the oxidative PTMs and quantitative multiplexed proteomics that take place in the mouse heart tissue during the first 48h after I/R. Surgically-induced MI was performed and animals were sacrificed at 10 min, 20 min, 40 min, 80 min, 2 hours, 6 hours, 12 hours, 24 hours, and 48 hours after reperfusion (n = 3 per group), and 3 mice were sacrificed with no intervention and used as control group. Whole hearts tissue was collected for proteomics evaluation.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

DISEASE(S): Myocardial Ischemia

SUBMITTER: Inmaculada Jorge

LAB HEAD: Jesús Vázquez Cobos

PROVIDER: PXD045759 | Pride | 2024-05-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	CC_MouseOxi_HF_TMT1_Fr1.msf	Msf
	CC_MouseOxi_HF_TMT1_Fr1.raw	Raw
	CC_MouseOxi_HF_TMT1_Fr1.txt	Txt
	CC_MouseOxi_HF_TMT1_Fr2.msf	Msf
	CC_MouseOxi_HF_TMT1_Fr2.raw	Raw

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Publications

Oxidative Post-translational Protein Modifications upon Ischemia/Reperfusion Injury.

Binek Aleksandra A Castans Celia C Jorge Inmaculada I Bagwan Navratan N Rodríguez José Manuel JM Fernández-Jiménez Rodrigo R Galán-Arriola Carlos C Oliver Eduardo E Gómez Mónica M Clemente-Moragón Agustín A Ibanez Borja B Camafeita Emilio E Vázquez Jesús J

Antioxidants (Basel, Switzerland) 20240116 1

While reperfusion, or restoration of coronary blood flow in acute myocardial infarction, is a requisite for myocardial salvage, it can paradoxically induce a specific damage known as ischemia/reperfusion (I/R) injury. Our understanding of the precise pathophysiological molecular alterations leading to I/R remains limited. In this study, we conducted a comprehensive and unbiased time-course analysis of post-translational modifications (PTMs) in the post-reperfused myocardium of two different anim ...[more]

PMID: 38247530

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