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The characterisation of protein and cellular binding in paediatric extracorporeal membrane oxygenation (ECMO) circuits using proteomics, immunofluorescence, and scanning electron microscopy

ABSTRACT: Extracorporeal membrane oxygenation (ECMO) has been increasingly applied to both adult and paediatric patients worldwide. However, continuous contact between blood and foreign surface of the ECMO circuit may contribute to haemostatic, inflammatory and other physiological disturbances during ECMO. Although previous studies have extensively investigated blood samples from patients on ECMO, the protein and cellular binding to the ECMO circuit has largely been overlooked as an additional factor that could contribute to differences in clinical outcomes. The detailed protocol described in this paper enables the comprehensive characterisation of protein and cellular binding in paediatric ECMO circuits, which could extend our knowledge of the pathophysiology of circuit binding and provide guidance for improved ECMO circuit design.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood

SUBMITTER: Laura Dagley

LAB HEAD: Prof Vera Ignjatovic

PROVIDER: PXD046085 | Pride | 2024-10-07

REPOSITORIES: Pride

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			Action	DRS
	DIANN_libfree.zip	Other
	P4439_02_1_1_5306.d.zip	Other
	P4439_03_1_1_5307.d.zip	Other
	P4439_04_1_1_5312.d.zip	Other
	P4439_06_1_1_5308.d.zip	Other

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