Dataset Information

Proteome of Corynebacterium glutamicum at different pH values

ABSTRACT: Recently, we established Corynebacterium glutamicum as a suitable production host for various bacteriocins including garvicin Q (GarQ). Here, we establish secretion of GarQ by C. glutamicum via the Sec translocon. At neutral pH, the cationic peptide is efficiently adsorbed to the negatively charged envelope of producer bacteria limiting availability of the bacteriocin in culture supernatants. Using a reporter strain and proteomic analyses, we identified HtrA, a protease associated with secretion stress, as another potential factor limiting GarQ production.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Corynebacterium Glutamicum Atcc 13032

SUBMITTER: Valentin Steier

LAB HEAD: Marco Oldiges

PROVIDER: PXD046499 | Pride | 2024-01-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Coryne_180216.fasta	Fasta
	Coryne_190506.group	Other
	pH_7_shift_pH_5_22h.1.1.1.wiff	Wiff
	pH_7_shift_pH_5_22h.1.1.1.wiff.scan	Wiff
	pH_7_shift_pH_5_22h.1.1.2.wiff	Wiff

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Publications

Garvicin Q: characterization of biosynthesis and mode of action.

Desiderato Christian K CK Hasenauer Katharina M KM Reich Sebastian J SJ Goldbeck Oliver O Holivololona Lalaina L Ovchinnikov Kirill V KV Reiter Alexander A Oldiges Marco M Diep Dzung B DB Eikmanns Bernhard J BJ Riedel Christian U CU

Microbial cell factories 20221111 1

Bacteriocins are ribosomally synthesized antimicrobial peptides, that either kill target bacteria or inhibit their growth. Bacteriocins are used in food preservation and are of increasing interest as potential alternatives to conventional antibiotics. In the present study, we show that Lactococcus petauri B1726, a strain isolated from fermented balsam pear, produces a heat-stable and protease-sensitive compound. Following genome sequencing, a gene cluster for production of a class IId bacterioci ...[more]

PMID: 36368990

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