Dataset Information

Proteome Analysis of Actinoplanes sp. SE50/110

ABSTRACT: The acarviose metabolite acarbose is an a glucosidase inhibitor produced by Actinoplanes sp. SE50/110. It is medically important because it is used in the treatment of type 2 diabetes. In this work a comprehensive proteome analysis of Actinoplanes sp. SE50/110 was carried out. The associated txt and RAW files were used for two different analyses and publications. While one study focused on a comparative analysis of Actinoplanes sp. SE50/110 to elucidate differences in the proteome cultures that were grown with either maltose or glucose, the other study applied spectral counting and analyzed only the maltose-grown cultures to determine the major proteins and their location in the cell. The txt files for the comparative data are labeled as "heavy_light" and of the spectral counting data as "light". Both datasets were derived from the same RAW files.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Actinoplanes Sp. (strain Atcc 31044 / Cbs 674.73 / Se50/110)

SUBMITTER: Andreas Otto

LAB HEAD: Andreas Otto

PROVIDER: PXD001497 | Pride | 2015-10-21

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	130326_o3_p4_AO_SWe_Maltose_cytosol_1_01.RAW	Raw
	130326_o3_p4_AO_SWe_Maltose_cytosol_1_02.RAW	Raw
	130326_o3_p4_AO_SWe_Maltose_cytosol_1_03.RAW	Raw
	130326_o3_p4_AO_SWe_Maltose_cytosol_1_04.RAW	Raw
	130326_o3_p4_AO_SWe_Maltose_cytosol_1_05.RAW	Raw

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Publications

Comprehensive proteome analysis of Actinoplanes sp. SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster.

Wendler Sergej S Otto Andreas A Ortseifen Vera V Bonn Florian F Neshat Armin A Schneiker-Bekel Susanne S Walter Frederik F Wolf Timo T Zemke Till T Wehmeier Udo F UF Hecker Michael M Kalinowski Jörn J Becher Dörte D Pühler Alfred A

Journal of proteomics 20150418

Acarbose is an α-glucosidase inhibitor produced by Actinoplanes sp. SE50/110 that is medically important due to its application in the treatment of type2 diabetes. In this work, a comprehensive proteome analysis of Actinoplanes sp. SE50/110 was carried out to determine the location of proteins of the acarbose (acb) and the putative pyochelin (pch) biosynthesis gene cluster. Therefore, a comprehensive state-of-the-art proteomics approach combining subcellular fractionation, shotgun proteomics and ...[more]

PMID: 25896738

Publication: 1/2

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