Dataset Information

Unraveling epigenetic changes in A. thaliana calli: Impact of HDAC inhibitors

ABSTRACT: The aim was to characterize epigenetic changes in histone proteins during callus formation from roots and shoots of Arabidopsis thaliana seedlings using mass spectrometry-based approach. Increased level of histone H3.3 variant was found to be the most prominent feature of 20-day-old calli, associated with chromatin relaxation. Methylation status in root- and shoot-derived calli reached the same level during long-term propagation, whereas differences in acetylation levels provided a long-lasting imprint of root and shoot origin. On the other hand, epigenetic signs of origin completely disappeared during 20 days of calli propagation in the presence of histone deacetylase inhibitors (HDACi), sodium butyrate and trichostatin A, although each HDACi affected the state of post-translational histone modifications in a specific manner.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell

SUBMITTER: Gabriela Lochmanova

LAB HEAD: Zbynek Zdrahal

PROVIDER: PXD046788 | Pride | 2024-01-26

REPOSITORIES: Pride

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Unraveling Epigenetic Changes in <i>A. thaliana</i> Calli: Impact of HDAC Inhibitors.

Pírek Pavlína P Kryštofová Karolína K Kováčová Ingrid I Kromerová Anna A Zachová Dagmar D Helia Ondřej O Panzarová Klára K Fajkus Jiří J Zdráhal Zbyněk Z Lochmanová Gabriela G Fojtová Miloslava M

Plants (Basel, Switzerland) 20231215 24

The ability for plant regeneration from dedifferentiated cells opens up the possibility for molecular bioengineering to produce crops with desirable traits. Developmental and environmental signals that control cell totipotency are regulated by gene expression via dynamic chromatin remodeling. Using a mass spectrometry-based approach, we investigated epigenetic changes to the histone proteins during callus formation from roots and shoots of <i>Arabidopsis thaliana</i> seedlings. Increased levels ...[more]

PMID: 38140504

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			Action	DRS
	1y-CTR_RH1.msf	Msf
	1y-CTR_RH1.raw	Raw
	1y-CTR_RH2.msf	Msf
	1y-CTR_RH2.raw	Raw
	1y-CTR_RH3.msf	Msf