Dataset Information

Probing Blood Plasma Protein Glycosylation with Infrared Spectroscopy

ABSTRACT: The health state of an individual is closely linked to the glycosylation patterns of their blood plasma proteins. However, obtaining this information requires cost- and time-efficient analytical methods. We put forward infrared spectroscopy, which allows label-free analysis of protein glycosylation, but so far has only been applied to analyses of individual proteins. Although spectral information does not directly provide the molecular structure of the glycans, it is sensitive to changes therein and covers all types of glycosidic linkages. Combining single-step ion exchange chromatography with infrared spectroscopy, we developed a workflow that enables separation and analysis of major protein classes in blood plasma. Our results demonstrate that infrared spectroscopy can identify different patterns and global levels of glycosylation of intact plasma proteins. To showcase the strengths and limitations of the proposed approach, we compare the glycoforms of human and bovine alpha-1-acid glycoproteins, which exhibit highly variable global levels of glycosylation. To further independently evaluate our conclusions, the glycan moieties of human alpha-1-acid glycoprotein were further analyzed using established glycomics workflow. Importantly, the chromatographic separation of blood plasma improves the detection of aberrant glycoforms of a given protein, as compared to infrared spectroscopy of bulk plasma. The presented approach allows time-efficient comparison of glycosylation patterns of multiple plasma proteins, opening new avenues for biomedical probing.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Christina Ludwig

LAB HEAD: Christina Ludwig

PROVIDER: PXD046796 | Pride | 2024-02-26

REPOSITORIES: Pride

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			Action	DRS
	BBM_736_P309_01_FRH_001.raw	Raw
	BBM_736_P309_01_FRH_002.raw	Raw
	BBM_736_P309_01_FRH_003.raw	Raw
	BBM_736_P309_01_FRH_004.raw	Raw
	BBM_736_P309_01_FRH_005.raw	Raw

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Probing Blood Plasma Protein Glycosylation with Infrared Spectroscopy.

Voronina Liudmila L Fleischmann Frank F Šimunović Jelena J Ludwig Christina C Novokmet Mislav M Žigman Mihaela M

Analytical chemistry 20240207

The health state of an individual is closely linked to the glycosylation patterns of his or her blood plasma proteins. However, obtaining this information requires cost- and time-efficient analytical methods. We put forward infrared spectroscopy, which allows label-free analysis of protein glycosylation but so far has only been applied to analysis of individual proteins. Although spectral information does not directly provide the molecular structure of the glycans, it is sensitive to changes the ...[more]

PMID: 38324652

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