Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of the human intestinal bacterium Eggerthella lenta on the liver proteome of gnotobiotic mice.

ABSTRACT: In this study, the minimal consortium Oligo-Mouse Microbiota (OMM)12 was used to study the function of Coriobacteriia under defined conditions in gnotobiotic mice. OMM12 mice with or without adding the dominant gut bacterium Eggerthella lenta were fed with diets varying in fat content or supplemented with primary bile acids. Changes in the host liver proteomes were studied using untargeted proteomics.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Kristin Schubert

LAB HEAD: Kristin Schubert

PROVIDER: PXD047016 | Pride | 2024-01-19

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	1_static.pdStudy	Other
	20211116_Uniprot_MusMusculus_REFSET_IK.fasta	Fasta
	ExperimentalDesign.xlsx	Xlsx
	Leber01_F1.raw	Raw
	Leber01_F2.raw	Raw

Items per page:

1 - 5 of 22

Similar Datasets

The gut bacterium Extibacter muris produces secondary bile acids and influences liver physiology in gnotobiotic mice - Proteomics results

Project description:In this study, mice were colonized from birth on with the minimal microbiota Oligo-MM with or without the addition of Extibacter muris. Extibacter muris is known to metabolize cholic acid to deoxycholic acid via 7α-dehydroxylation. The impact of Extibacter muris colonization on the liver proteome of mice under different diets was investigated using untargeted proteomics.

2021-01-04 | PXD019973 | Pride

Effect of rosiglitazone on adipose gene expression in HSL knockout mice with different diet treatment

Project description:To test whether HSL is required for the supply of intrinsic ligands for PPARg for normal adipose differentiation, HSL-/- and wild type (WT) littermates were fed normal chow (NC) and high fat (HF) diets supplemented with or without rosiglitazone (200 mg/kg) for 16 weeks. The expression of diabetes related genes in the WAT of the animals was analyzed using Oligo GEArray Mouse Diabetes Microarrays (OMM-023, SABiosciences, Frederick, MD). Together with other biochemical and physiological data, our results suggest that one of the mechanisms by which HSL modulates adipose metabolism is by providing intrinsic ligands for PPARg. To examine the changes in gene expression in WAT of WT and HSL-/- mice with rosiglitazone and diet treatment, RNA isolated from 4 animals of each group were used for studies of gene expression profiles using Oligo GEArray Mouse Diabetes Microarrays (OMM-023, SABiosciences, Frederick, MD). Data were analyzed using GEArray Expression Analysis Suite (SABiosciences, Frederick, MD) and SAM programs to comprehensively evaluate the differential gene expression of the various samples.

2012-03-18 | E-GEOD-20588 | biostudies-arrayexpress

Effect of rosiglitazone on adipose gene expression in HSL knockout mice with different diet treatment

2012-03-18 | GSE20588 | GEO

A chaperone-assisted mechanism of outer mitochondrial membrane TOM70 insertion protective against diet-induced obesity

Project description:Outer mitochondrial membrane (OMM) proteins communicate with the cytosol and other organelles including the endoplasmic reticulum (ER). This communication is important in thermogenic adipocytes to increase energy expenditure that controls body temperature and weight. However, the regulatory mechanisms of OMM protein insertion are poorly understood. Herein, stress-induced cytosolic chaperone PPID (peptidyl-prolyl isomerase D/cyclophilin 40/Cyp40) drives OMM insertion of the mitochondrial import receptor TOM70 regulating body temperature and weight in obese mice, and respiratory/thermogenic function in brown adipocytes. PPID PPIase activity and C-terminal tetratricopeptide repeats (TPR), which show specificity towards TOM70 core and C-tail domains, facilitate OMM insertion. Our results provide an unprecedented role for ER-stress-activated chaperones in controlling energy metabolism through a selective OMM protein insertion mechanism with implications in adaptation to cold temperatures and high-calorie diets.

2024-10-03 | PXD053607 | Pride

A chaperone-assisted mechanism of outer mitochondrial membrane TOM70 insertion protective against diet-induced obesity

2024-10-02 | PXD055263 | Pride

A chaperone-assisted mechanism of outer mitochondrial membrane TOM70 insertion protective against diet-induced obesity

2024-10-02 | PXD054182 | Pride

Proteomics analysis of bile acids modulation on rat colonic expression of water, electrolyte and drug transporters

Project description:Bile acid diarrhoea is a chronic condition with increased delivery of bile acids to the colon causing diarrhoea. In this study we used a rodent model of bile acid malabsorption to investigate the general impact on increased levels of bile acids on mRNA- and protein-expression in colon epithelial cells. Twelve male Wistar Munich rats were housed in metabolic cages and fed control or bile acid-mixed (1% w/w) diets for ten days. After euthanasia, colonic epithelial cells were isolated using Ca2+ chelation and processed for mass spectrometry-based proteomics to quantify changes in protein expressions.

2021-11-03 | PXD025915 | Pride

Project description:OMM-12 and OMM-19 colonisation across gut sites

| PRJNA807946 | ENA

The Microbial Metabolite Deoxycholic Acid Modulates Cholesterol Metabolism in the Germ-Free Mouse

Project description:Deoxycholic acid (DCA) is a secondary bile acid produced by a small number of commensal species of bacteria present in the mammalian gut. Elevated DCA concentration correlates with disease states including colon cancer and cholesterol gallstones, but the associated mechanisms are not fully understood. Both primary and secondary bile acids are also capable of affecting gene expression through nuclear receptors such as FXR. To better understand the impact of a commensal-derived secondary bile acid on host metabolism we fed DCA to germ-free (GF) mice, which normally lack DCA, and compared the hepatic transcriptomes of bile acid fed GF mice to GF mice receiving a control diet, as well as to those of conventionally housed control animals. Interestingly, the feeding of DCA to GF mice, but not the feeding of cholic acid (CA) from which DCA is derived, results in an up-regulation of genes of cholesterol biosynthetic pathways. GF mice normally have elevated hepatic cholesterol compared to conventionally housed mice. Despite increase in the expression of cholesterol biosynthetic genes, the DCA fed GF mice showed a markedly decreased level of hepatic cholesterol equivalent to the hepatic cholesterol concentration of conventionally colonized animals. Total cholesterol in the serum was unaffected by DCA, but there was a decrease in the HDL lipoprotein fraction as well as an increase in the non-HDL lipoprotein fraction of the serum cholesterol. DCA, but not CA, is sufficient to modulate host lipoprotein metabolism. Taken together, these results suggests that a minor component of the gut microbiome has a significant impact on cholesterol homeostasis through secondary metabolism of bile acids and suggests a possible therapeutic intervention route through the microbial metabolic pathways. two mouse strains, three diets, one time point

2015-01-08 | E-GEOD-34730 | biostudies-arrayexpress

Transcriptome Analysis of Common Carp (Cyprinus carpio) Fed with Animal and Plant Diets

Project description:purpose?To elucidate the relationship of utilization different type of diets in fish method?enzyme activity determination and transcriptome sequencing were performed in common carp fed with single animal diet group (group AD), plant diet group (group PD) and mix diets group (group MD). Group MD as control group results? 916 and 1296 differentially expressed genes were identified between group AD vs MD and PD vs MD. Protein digestion and absorption, bile secretion, hematopoietic cell lineage and intestinal immune network for IgA production pathways were significantly differentially expressed between common carp fed with single type of diet and mix diets. Conclusion?common carp fed with mix diets had stronger immunity than common carp fed with single type of diets.

2019-01-19 | GSE125366 | GEO