Proteomics

Dataset Information

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A Photocrosslinking Probe to Capture the Substrates of Caseinolytic Protease P


ABSTRACT: Protein homeostasis in bacteria is regulated by proteases such as the tetradecameric caseinolytic protease P (ClpP). Although substrates of ClpP have been successfully deciphered in genetically engineered cells, methods which directly trap processed proteins within native cells remain elusive. Here, we introduce an in situ trapping strategy which utilizes trifunctional probes that bind to the active site serine of ClpP and capture adjacent substrates with an attached photocrosslinking moiety. After enrichment using an alkyne handle, substrate deconvolution by mass spectrometry (MS) is performed. We show that our two traps bind substoichiometrically to ClpP, retain protease activity, exhibit unprecedented selectivity for Staphylococcus aureus ClpP in living cells and capture numerous known and novel substrates. The exemplary validation of trapped hits using a targeted proteomics approach confirmed the fidelity of this technology. In conclusion, we provide a novel chemical platform suited for the discovery of serine protease substrates beyond genetic engineering.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive HF

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Nina Bach  

LAB HEAD: Stephan Sieber

PROVIDER: PXD047274 | Pride | 2024-10-04

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20200506_TG_V145_D1.raw Raw
20200506_TG_V145_D2.raw Raw
20200506_TG_V145_D3.raw Raw
20200506_TG_V145_T11.raw Raw
20200506_TG_V145_T12.raw Raw
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Publications

A Photocrosslinking Probe to Capture the Substrates of Caseinolytic Protease P.

Gronauer Thomas F TF   Eck Laura K LK   Ludwig Christina C   Sieber Stephan A SA  

Angewandte Chemie (International ed. in English) 20240729


Protein homeostasis in bacteria is regulated by proteases such as the tetradecameric caseinolytic protease P (ClpP). Although substrates of ClpP have been successfully deciphered in genetically engineered cells, methods which directly trap processed proteins within native cells remain elusive. Here, we introduce an in situ trapping strategy which utilizes trifunctional probes that bind to the active site serine of ClpP and capture adjacent substrates with an attached photocrosslinking moiety. Af  ...[more]

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