Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion, Q Exactive HF
ORGANISM(S): Staphylococcus Aureus
SUBMITTER: Nina Bach
LAB HEAD: Stephan Sieber
PROVIDER: PXD047274 | Pride | 2024-10-04
REPOSITORIES: Pride
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Gronauer Thomas F TF Eck Laura K LK Ludwig Christina C Sieber Stephan A SA
Angewandte Chemie (International ed. in English) 20240729
Protein homeostasis in bacteria is regulated by proteases such as the tetradecameric caseinolytic protease P (ClpP). Although substrates of ClpP have been successfully deciphered in genetically engineered cells, methods which directly trap processed proteins within native cells remain elusive. Here, we introduce an in situ trapping strategy which utilizes trifunctional probes that bind to the active site serine of ClpP and capture adjacent substrates with an attached photocrosslinking moiety. Af ...[more]