Proteomics

Dataset Information

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APEX2 mediated proximity labeling to investigate the proximal proteome of mitochondria tethered ULK1 in wild type and fip200 knock down cells

ABSTRACT: The object of this project is to investigate the proximal proteome of ULK1 during mitophagy and the effects of a FIP200 knock down using the APEX2-mediated proximity labeling system. APEX2-ULK1 or APEX2 was fused to 2xFKBP-myc and stably expressed in a cell line stably expressing a truncated FIS1-FRB fusion protein. Treatment with rapalog induces the stable interaction between FRB and FKBP, resulting in tethering of FKBPx2-myc-APEX2-ULK1 or FKBPx2-myc-APEX2 to the outer mitochondrial membrane.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293t Cell, Cell Culture

SUBMITTER: David Hollenstein  

LAB HEAD: Claudine Kraft

PROVIDER: PXD047277 | Pride | 2024-10-12

REPOSITORIES: pride

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Dataset's files

Source:
Action DRS
2023.03_UP000005640_9606_Homo_sapiens_1protein_per_gene.fasta Fasta
20230810_E1_NEO3_Babic_Kraft_MPLGmbH_Academic_MPLID23159_s10_41_23p.raw Raw
20230810_E1_NEO3_Babic_Kraft_MPLGmbH_Academic_MPLID23159_s11_42_19p.raw Raw
20230810_E1_NEO3_Babic_Kraft_MPLGmbH_Academic_MPLID23159_s12_43_18p.raw Raw
20230810_E1_NEO3_Babic_Kraft_MPLGmbH_Academic_MPLID23159_s13_51_24p.raw Raw
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Publications

Autophagy is a key cellular quality control mechanism. Nutrient stress triggers bulk autophagy, which nonselectively degrades cytoplasmic material upon formation and liquid-liquid phase separation of the autophagy-related gene 1 (Atg1) complex. In contrast, selective autophagy eliminates protein aggregates, damaged organelles and other cargoes that are targeted by an autophagy receptor. Phase separation of cargo has been observed, but its regulation and impact on selective autophagy are poorly u  ...[more]

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