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APEX2 mediated proximity labeling to investigate the proximal proteome of mitochondria tethered ULK1 in wild type and fip200 knock down cells

ABSTRACT: The object of this project is to investigate the proximal proteome of ULK1 during mitophagy and the effects of a FIP200 knock down using the APEX2-mediated proximity labeling system. APEX2-ULK1 or APEX2 was fused to 2xFKBP-myc and stably expressed in a cell line stably expressing a truncated FIS1-FRB fusion protein. Treatment with rapalog induces the stable interaction between FRB and FKBP, resulting in tethering of FKBPx2-myc-APEX2-ULK1 or FKBPx2-myc-APEX2 to the outer mitochondrial membrane.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293t Cell, Cell Culture

SUBMITTER: David Hollenstein

LAB HEAD: Claudine Kraft

PROVIDER: PXD047277 | Pride | 2024-10-12

REPOSITORIES: Pride

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			Action	DRS
	2023.03_UP000005640_9606_Homo_sapiens_1protein_per_gene.fasta	Fasta
	20230810_E1_NEO3_Babic_Kraft_MPLGmbH_Academic_MPLID23159_s10_41_23p.raw	Raw
	20230810_E1_NEO3_Babic_Kraft_MPLGmbH_Academic_MPLID23159_s11_42_19p.raw	Raw
	20230810_E1_NEO3_Babic_Kraft_MPLGmbH_Academic_MPLID23159_s12_43_18p.raw	Raw
	20230810_E1_NEO3_Babic_Kraft_MPLGmbH_Academic_MPLID23159_s13_51_24p.raw	Raw

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