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Mass Spectrometry of FAPS sorted ELVAs by an alternative sorting strategy

ABSTRACT: We discovered that mouse oocytes contain novel non-membrane-bound organelles which we named EndoLysosomal Vesicular Assemblies (ELVAs). ELVAs contain endolysosomal and autophagy vesicles as well as Proteasomes held together by a matrix protein. To discover the matrix protein holding ELVAs together we subjected mouse oocyte with fluorescently labelled ELVAs to mechanical lysis followed by Fluorescence-Activated Particle Sorting to enrich for ELVAs. Enriched ELVAs were then subjected to proteomics.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Oocyte

SUBMITTER: Gabriele Zaffagnini

LAB HEAD: Elvan Böke

PROVIDER: PXD047716 | Pride | 2024-02-20

REPOSITORIES: Pride

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			Action	DRS
	2020MQ038_GAZA_001_01_90pto.raw	Raw
	2020MQ038_GAZA_002_01_90pto.raw	Raw
	2020MQ038_GAZA_003_01_90pto.raw	Raw
	2020MQ038_GAZA_004_01_90pto.raw	Raw
	2020MQ038_GAZA_005_01_90pto.raw	Raw

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Project description:We discovered that mouse oocytes contain novel non-membrane-bound organelles which we named EndoLysosomal Vesicular Assemblies (ELVAs). ELVAs contain endolysosomal and autophagy vesicles as well as Proteasomes held together by a matrix protein. To discover the matrix protein holding ELVAs together we subjected mouse oocyte with fluorescently labelled ELVAs to mechanical lysis followed by Fluorescence-Activated Particle Sorting to enrich for ELVAs. Enriched ELVAs were then subjected to proteomics.

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