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Analysis of the impact of cellular miRNA signatures on the profiles of circulating miRNA biomarkers (fractionation data)

ABSTRACT: Circulating miRNAs are an emerging class of biomarkers correlating their specific expression patterns to disease states. A considerable proportion of hematopoietically-derived miRNAs are present in plasma with the ability to confound the signature of true circulating miRNA species. We use microarray analysis to catalogue a list of 313 haemotopoetic miRNAs and analyze expression profiles of cell-free miRNAs in individual plasma fractions after calibrating for cellular miRNA signals. Comprehensive global maps of bona fide circulating miRNA species are presented, and inter-individual variability and gender-specific expression is explored in populations of healthy individuals. Healthy Caucasian individuals were used to segregate blood into 8 subfractions: white blood cells (WBC)/buffy coat, leukocytes, red blood cells (red blood cells (RBC)), cloudy supernatant (CS), supernatant 1 (S1), supernatant 2 (S2), pellet 1 (P1) and pellet 2 (P2) through differential centrifugation to understand the proportion of cellular miRNAs in each category.

ORGANISM(S): Homo sapiens

SUBMITTER: Radha Duttagupta 

PROVIDER: E-GEOD-27230 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Impact of cellular miRNAs on circulating miRNA biomarker signatures.

Duttagupta Radha R   Jiang Rong R   Gollub Jeremy J   Getts Robert C RC   Jones Keith W KW  

PloS one 20110617 6

Effective diagnosis and surveillance of complex multi-factorial disorders such as cancer can be improved by screening of easily accessible biomarkers. Highly stable cell free Circulating Nucleic Acids (CNA) present as both RNA and DNA species have been discovered in the blood and plasma of humans. Correlations between tumor-associated genomic/epigenetic/transcriptional changes and alterations in CNA levels are strong predictors of the utility of this biomarker class as promising clinical indicat  ...[more]

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