Tardigrades dramatically upregulate DNA repair pathway genes in response to ionizing radiation

ABSTRACT: Tardigrades can survive remarkable doses of ionizing radiation, up to about 1000 times the lethal dose for humans. How they do so is incompletely understood. We found that the tardigrade Hypsibius exemplaris suffers DNA damage upon gamma irradiation, but damage is repaired. We show that tardigrades have a specific and robust response to ionizing radiation: irradiation induces a rapid, dramatic upregulation of many DNA repair genes. By expressing tardigrade genes in bacteria, we validate that increased expression of some repair genes can suffice to increase radiation tolerance. We show that at least one such gene is necessary for tardigrade radiation tolerance. Tardigrades’ ability to sense ionizing radiation and massively upregulate specific DNA repair pathway genes may represent an evolved solution for maintaining DNA integrity.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Hypsibius Exemplaris

SUBMITTER: Leslie Hicks  

LAB HEAD: Leslie Hicks

PROVIDER: PXD047724 | Pride | 2024-12-13

REPOSITORIES: pride