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Differences between static supernatant-, static biofilm- and flow biofilm-cells of two Lactiplantibacillus plantarum strains.

ABSTRACT: This study was conducted to analyze phenotypic and proteomic differences of two Lactiplantibacillus plantarum strains (WCFS1, model strain from human saliva, and CIP104448, stool isolate) when a biofilm was produced under static conditions (well researched), or with the addition of flow (novel).

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Lactiplantibacillus Sp. Lactobacillus Plantarum Wcfs1

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD047751 | Pride | 2024-06-03

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MQresult_CIP_TA373-387.zip	Other
	MQresult_WCFS_TA370-384.zip	Other
	TA370_WCFS_S_Sup.raw	Raw
	TA371_WCFS_S_Bio.raw	Raw
	TA372_WCFS_F_Bio.raw	Raw

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1 - 5 of 21

Publications

Phenotypic and proteomic differences in biofilm formation of two <i>Lactiplantibacillus plantarum</i> strains in static and dynamic flow environments.

Huijboom Linda L Rashtchi Parisa P Tempelaars Marcel M Boeren Sjef S van der Linden Erik E Habibi Mehdi M Abee Tjakko T

Biofilm 20240422

<i>Lactiplantibacillus plantarum</i> is a Gram-positive non-motile bacterium capable of producing biofilms that contribute to the colonization of surfaces in a range of different environments. In this study, we compared two strains, WCFS1 and CIP104448, in their ability to produce biofilms in static and dynamic (flow) environments using an in-house designed flow setup. This flow setup enables us to impose a non-uniform flow velocity profile across the well. Biofilm formation occurred at the bott ...[more]

PMID: 38706985

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Fermentation of soy juice by Lactiplantibacillus plantarum confers a yogurt-like texture, flavours and health-promoting metabolites

Project description:The aim of the study was to decipher metabolisms responsible (i) for the peculiar adaptation of L. plantarum during soy juice fermentation and (ii) for the release of aroma compounds, amino and short-chain fatty acid, and metabolites with health-promoting properties in soy yogurt. The strategy was the sequencing and annotation of a strain (L. plantarum CIRM-BIA777, PRJEB77707) able to degrade galacto- oligosaccharides, the sampling of soy yogurt, RNA-seq to identify differentially expressed genes of L. plantarum and corresponding metabolisms throughout the kinetics of fermentation. Acids and volatile compounds were also quantified.

2024-08-18 | E-MTAB-14345 | biostudies-arrayexpress

Interaction between Lactiplantibacillus plantarum RX-8 and Wickerhamomyces anomalus Y-5 under co-culture by proteomics analysis

Project description:In present study, it was found that the co-culture of Wickerhamomyces anomalus Y-5 and L. plantarum RX-8 could enhance bacteriocin production. To analyze the interaction between W. anomalus Y-5 and L. plantarum RX-8, a quantitative proteomic approach was used to analyze and compare the proteome in L. plantarum RX-8 and W. anomalus Y-5 under mono-culture and co-culture. In total, 339 differently expressed proteins (DEPs) were screened in comparison of L. plantarum RX-8 under mono-culture and co-culture, 645 proteins of W. anomalus Y-5 changed in mono-culture and co-culture. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that DEPs participated in various metabolic pathways such as PTS system, glycolysis, galactose metabolism, glutamate, aspartate, arginine and cysteine metabolism etc. These pathways were related to inducing mechanism on improving bacteriocin production by co-culture. Quantitative proteomic analysis-based strategies can therefore provide further evidence for new regulated targets to improve the production of bacteriocins.

2022-12-04 | PXD038315 | Pride

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Project description:the human in vivo transcriptional response of small intestinal mucosa to Lactobacillus plantarum

2008-10-17 | E-MEXP-1328 | biostudies-arrayexpress

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Transcriptome analyses of aggregating Lactiplantibacillus plantarum

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2024-01-26 | PXD042175 | Pride

Pig SISP jejunal segments: Lactobacillus plantarum and msa/srt mutant strains vs control, perfusion 4 or 8 hours

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Extracellular vesicles of Lactiplantibacillus plantarum PCM 2675

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2024-11-07 | PXD048874 | Pride

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