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Porcine pancreas tissue-derived decellularized extracellular matrix LC-MS/MS

ABSTRACT: The protein composition of porcine pancreatic tissue-derived decellularized extracellular matrix was identified.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Pancreas

SUBMITTER: Myungji Kim

LAB HEAD: Myungji Kim

PROVIDER: PXD047892 | Pride | 2025-01-27

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20190227_PIG_PdECM_E01.msf	Msf
	20190227_PIG_PdECM_E01.pdResult	Other
	20190227_PIG_PdECM_E01.raw	Raw
	pdecm.pdStudy	Other

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Publications

Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets.

Kim Myungji M Cho Seungyeun S Hwang Dong Gyu DG Shim In Kyong IK Kim Song Cheol SC Jang Jiwon J Jang Jinah J

Nature communications 20250207 1

Pancreatic islets are densely packed cellular aggregates containing various hormonal cell types essential for blood glucose regulation. Interactions among these cells markedly affect the glucoregulatory functions of islets along with the surrounding niche and pancreatic tissue-specific geometrical organization. However, stem cell (SC)-derived islets generated in vitro often lack the three-dimensional extracellular microenvironment and peri-vasculature, which leads to the immaturity of SC-derived ...[more]

PMID: 39920133

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Project description:In this study we have employed RNA seq on ten different tissues including four brain tissues from two boars to gain a understanding of the differential variations in transcriptional profiles for these tissues consisting of occipital cortex, frontal cortex, hypothalamus and cerebellum along with such diverse tissues as heart, spleen, liver, kidney, lung and musculus longissimus dorsi. This has enabled us to perform comparative gene expression analysis of brain regions versus non-brain tissues along with inter-brain tissue comparisons. Hence, we have tested for differentially expressed genes and isoforms, differential splicing, transcription start sites (TSS), and differential promoter usage between all ten porcine tissues.

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On the characterisation of the porcine gland-specific salivary proteome using MALDI-TOF/TOF MS and Q-Exactive orbitrap MS/MS

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