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BioID-MS of TBX3-proximal proteins in HEK293T cells

ABSTRACT: Wnt signaling orchestrates gene expression in a plethora of processes during development and adult cell homeostasis via the action of nuclear b-catenin. Yet, little is known about how b-catenin generates context-specific transcriptional outcomes. Addressing this knowledge gap is pivotal since neoplasia of the colorectal epithelium begins with aberrant Wnt/b-catenin signaling and broadly targeting the Wnt pathway can lead to undesirable consequences. We have previously identified the developmental transcription factor TBX3 as a tissue-specific component of the Wnt/b-catenin nuclear complex during mouse forelimb development. Here, we screen for TBX3-interacting nuclear proteins in HEK293T cells by performing BioID followed by mass spectrometry.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: Simon Söderholm

LAB HEAD: Claudio Cantù

PROVIDER: PXD047899 | Pride | 2025-04-22

REPOSITORIES: Pride

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			Action	DRS
	CHIR_Ctrl_BirA_1.mgf	Mgf
	CHIR_Ctrl_BirA_1.msf	Msf
	CHIR_Ctrl_BirA_1.raw	Raw
	CHIR_Ctrl_BirA_1_Proteins.txt	Txt
	CHIR_Ctrl_BirA_2.mgf	Mgf

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