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Autophosphorylation of Tousled-like kinases (TLK1 and TLK2) regulates recruitment to damaged chromatin via PCNA interaction (Phospho-TMT analysis of TLK1)

ABSTRACT: PhosphoTMT experiment on HEK293T cells transiently transfected with either SFB-TLK1, SFB-TLK2, or siRNAs targeting TLK1, TLK2, or TLK1 and TLK2. Data represents 4 biological replicates.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Kirk West

LAB HEAD: Justin W. Leung

PROVIDER: PXD047966 | Pride | 2025-01-02

REPOSITORIES: pride

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			Action	DRS
	Leung_TMT1_Phos_082619_SF1.raw	Raw
	Leung_TMT1_Phos_082619_SF10.raw	Raw
	Leung_TMT1_Phos_082619_SF11.raw	Raw
	Leung_TMT1_Phos_082619_SF12.raw	Raw
	Leung_TMT1_Phos_082619_SF13.raw	Raw

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Publications

Autophosphorylation of the Tousled-like kinases TLK1 and TLK2 regulates recruitment to damaged chromatin via PCNA interaction.

West Kirk L KL Nguyen Tram T N TTN Tengler Kyle A KA Kreiling Natasha N Raney Kevin D KD Ghosal Gargi G Leung Justin W JW

Nucleic acids research 20241227

Tousled-like kinases 1 and 2 (TLK1 and 2) are cell cycle-regulated serine/threonine kinases that are involved in multiple biological processes. Mutation of TLK1 and 2 confer neurodegenerative diseases. Recent studies demonstrate that TLK1 and 2 are involved in DNA repair. However, there is no direct evidence that TLK1 and 2 function at DNA damage sites. Here, we show that both TLK1 and TLK2 are hyper-autophosphorylated at their N-termini, at least in part, mediated by their homo- or hetero- dime ...[more]

PMID: 39727191

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