Dataset Information

Proteomic Biomarkers for Leishmanial Extracellular Vesicles

ABSTRACT: Despite their clinical relevance and role in parasitic virulence, exosomes produced by the protozoan parasite Leishmania lack specific proteomic biomarkers. Herein, we utilized utilized serial differential ultracentrifugation to separate three distinct fractions of Leishmania-derived extracellular vesicles and validated their properties using nanoparticle tracking analysis and transmission electron microscopy. Proteomic analysis was then used to assess their protein contentand identify novel biomarkers.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Leishmania Major

TISSUE(S): Cell Culture

DISEASE(S): Leishmaniasis

SUBMITTER: Andrea Lafleur

LAB HEAD: Dr. Martin Olivier

PROVIDER: PXD048015 | Pride | 2024-06-22

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	VL_20170518_OLI_01.mzid.gz	Mzid
	VL_20170518_OLI_01.mzid_VL_20170518_OLI_01.MGF	Mzid
	VL_20170518_OLI_01.raw	Raw
	VL_20170518_OLI_02.mzid.gz	Mzid
	VL_20170518_OLI_02.mzid_VL_20170518_OLI_02.MGF	Mzid

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Publications

Identification of potential novel proteomic markers of <i>Leishmania</i> spp.-derived exosomes.

da Silva Lira Filho Alonso A Lafleur Andrea A Marcet-Palacios Marcelo M Olivier Martin M

Frontiers in cellular and infection microbiology 20240219

<h4>Introduction</h4>Extracellular vesicles (EVs) are heterogenous cell-derived membrane-bound structures which can be subdivided into three distinct classes according to distinct morphological characteristics, cellular origins, and functions. Small EVs, or exosomes, can be produced by the protozoan parasite <i>Leishmania</i> through the evolutionarily conserved ESCRT pathway, and act as effectors of virulence and drivers of pathogenesis within mammalian hosts. Techniques for the identification ...[more]

PMID: 38440791

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