Proteomics

Dataset Information

0

Adaptive laboratory evolution of Clostridium autoethanogenum to metabolize CO2 and H2 enhances growth rates in chemostat and unravels proteome and metabolome alterations


ABSTRACT: Gas fermentation of CO₂ and H₂ is an attractive means to sustainably produce fuels and chemicals. Clostridium autoethanogenum is a model organism for industrial CO-to-ethanol and presents an opportunity for CO₂-to-ethanol processes. As we have previously characterized its CO₂/H₂ chemostat growth, here we use adaptive laboratory evolution (ALE) with the aim of improving growth with CO₂/H₂. Seven ALE lineages were generated, all with improved specific growth rates. Developed with 2% CO supplementation of CO₂/H₂, Evolved lineage D has the highest ethanol/acetate of ALE lineages when fermenting CO₂/H₂. Chemostat comparison against the parental strain shows no change in acetate or ethanol production, while Evolved D could achieve a higher maximum dilution rate. Complete multi-omics analyses at steady-state revealed that although Evolved D has widespread proteome changes, intracellular metabolites prevent phenotype shifts. Yet, we observe numerous insights to CO₂/H₂ metabolism via these multi-omics results and link these to mutations, suggesting novel targets for metabolic engineering.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Clostridium Autoethanogenum

SUBMITTER: James Heffernan  

LAB HEAD: Esteban Marcellin

PROVIDER: PXD048075 | Pride | 2024-05-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
202007016_T2_005.raw Raw
202007016_T2_008.raw Raw
202007016_T2_011.raw Raw
202007016_T2_014.raw Raw
202007016_T2_017.raw Raw
Items per page:
1 - 5 of 8

Similar Datasets

2024-05-09 | PXD048143 | Pride
2007-08-31 | GSE8898 | GEO
2018-03-05 | PXD008367 | Pride
2020-12-03 | PXD006631 | Pride
2011-09-05 | E-GEOD-30535 | biostudies-arrayexpress
2022-07-16 | PXD031078 | Pride
2008-06-16 | E-GEOD-8898 | biostudies-arrayexpress
2024-06-12 | PXD047330 | Pride
2021-12-31 | GSE152214 | GEO
| PRJNA642834 | ENA