Dataset Information

Characterization of fibrosis and senescence of human omental peritoneal mesothelial cells

ABSTRACT: Primary human omental mesothelial cells from multiple donors in a young (=normal) phase and senescent phase were treated with or without TGF-ß to induce fibrosis. Senescence was induced by up to 9 passages and confirmed by stopped cell division and positive senescence-associated β-galactosidase staining.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Mesothelial Cell, Peritoneum

SUBMITTER: Klaus Kratochwill

LAB HEAD: Dr Klaus Kratochwill

PROVIDER: PXD048090 | Pride | 2024-06-22

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	220304_EC_KK_123-P12197-Run1.msf	Msf
	220305_EC_KK_123-P12198-Run2.msf	Msf
	EC_KK_123-M1020-A01-O554-P12197-1.raw	Raw
	EC_KK_123-M1020-A02-O554-P12197-1.raw	Raw
	EC_KK_123-M1020-A03-O554-P12197-1.raw	Raw

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Publications

Effect of cellular senescence on the response of human peritoneal mesothelial cells to TGF-β.

Kawka Edyta E Herzog Rebecca R Ruciński Marcin M Malińska Agnieszka A Unterwurzacher Markus M Sacnun Juan Manuel JM Wagner Anja A Kowalska Katarzyna K Jopek Karol K Kucz-Chrostowska Agata A Kratochwill Klaus K Witowski Janusz J

Scientific reports 20240603 1

Transforming growth factor β (TGF-β) is implicated in both mesothelial-to-mesenchymal transition (MMT) and cellular senescence of human peritoneal mesothelial cells (HPMCs). We previously showed that senescent HPMCs could spontaneously acquire some phenotypic features of MMT, which in young HPMCs were induced by TGF-β. Here, we used electron microscopy, as well as global gene and protein profiling to assess in detail how exposure to TGF-β impacts on young and senescent HPMCs in vitro. We found t ...[more]

PMID: 38830931

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