Dataset Information

Human cholangiocarcinoma LC-MS/MS

ABSTRACT: We studied the effect of Ser30 with or without O-GlcNAc modification on K18 protein and its co-immunoprecipitated proteins in cholangiocarcinoma cells.The lysates from HuCCT1 shK18 stable cell line transfected with FLAG-K18-WT or FLAG-K18-S30A were captured with anti-FLAG beads, and then subjected to in-gel trypsin digestion and LC-MS/MS analysis.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Cholangiocarcinoma

SUBMITTER: xiangfeng Meng

LAB HEAD: Xiangfeng Meng

PROVIDER: PXD048144 | Pride | 2024-06-22

REPOSITORIES: Pride

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O-GlcNAcylation Facilitates the Interaction between Keratin 18 and Isocitrate Dehydrogenases and Potentially Influencing Cholangiocarcinoma Progression.

Meng Xiangfeng X Zhou Yue Y Xu Lei L Hu Limu L Wang Changjiang C Tian Xiao X Zhang Xiang X Hao Yi Y Cheng Bo B Ma Jing J Wang Lei L Liu Jialin J Xie Ran R

ACS central science 20240423 5

Glycosylation plays a pivotal role in the intricate landscape of human cholangiocarcinoma (CCA), actively participating in key pathophysiological processes driving tumor progression. Among the various glycosylation modifications, O-linked β-N-acetyl-glucosamine modification (O-GlcNAcylation) emerges as a dynamic regulator influencing diverse tumor-associated biological activities. In this study, we employed a state-of-the-art chemical proteomic approach to analyze intact gly ...[more]

PMID: 38799671

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Project description:Transcriptomic profiling Background Cholangiocarcinoma accounts for 5-10% of primary hepatic cancers. The etiology is unclear and patients are often diagnosed without risk factors. Resection is the only curative treatment although patients frequently remain undiagnosed until advanced stage of disease. Methods To construct molecular classification of cholangiocarcinoma, we profiled the transcriptomes of 104 freshly-frozen tumors and 59 matched non-cancerous livers obtained from Australia, Europe and the United States. We also performed mutational analysis of KRAS, EGFR and BRAF, and used laser-capture microdissection to obtain independent gene expression profiles for epithelial and stromal compartments in a subset of tumors. The selected target genes were validated by western blotting and immunohistochemistry. Results Transcriptomic profiling classified cholangiocarcinoma into two distinct subclasses defined by survival (P<0.0007) and early recurrence (P<0.001). Applying leave-one-out cross-validation, we optimized the prognostic classifier to 238 genes which were positively enriched in the epithelial tumor compartment. A deregulated HER2 network was associated with the epithelial compartment which also showed a frequent overexpression of Ki67, EGFR, MET and pRPS6 whereas inflammatory cytokines were enriched in tumor stroma specifically in patients with poor prognosis. KRAS mutations were found in 24.6% of patients with poor disease outcome. Conclusion Our study presents new insights into pathogenesis of cholangiocarcinoma and stratification of the patients according to survival and recurrence. Identification of a subgroup of patients among the poor prognostic cohort characterized by KRAS mutations and oncogenic-addiction may provide a novel therapeutic opportunity for this treatment-refractory malignancy. Profiling of individual cholangiocarcinomas and non-cancerous matched surrounding livers using normal bile ducts as reference

			Action	DRS
	K18-S30A-1.raw	Raw
	K18-S30A-2.raw	Raw
	K18-S30A-3.raw	Raw
	K18-WT-1.raw	Raw
	K18-WT-2.raw	Raw

Dataset Information

Human cholangiocarcinoma LC-MS/MS

Dataset's files

Publications

<i>O</i>-GlcNAcylation Facilitates the Interaction between Keratin 18 and Isocitrate Dehydrogenases and Potentially Influencing Cholangiocarcinoma Progression.

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Dataset Information

Human cholangiocarcinoma LC-MS/MS

Dataset's files

Publications

&lt;i&gt;O&lt;/i&gt;-GlcNAcylation Facilitates the Interaction between Keratin 18 and Isocitrate Dehydrogenases and Potentially Influencing Cholangiocarcinoma Progression.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

<i>O</i>-GlcNAcylation Facilitates the Interaction between Keratin 18 and Isocitrate Dehydrogenases and Potentially Influencing Cholangiocarcinoma Progression.