Immunomodulatory, antioxidant, and potential anticancer activity of the polysaccharides of the fungus Fomitiporia chilensis

ABSTRACT: Fomitiporia species have aroused the interest of numerous investigations that reveal their biological activity and medicinal potential. The present investigation shows the antioxi-dant, anticancer, and immunomodulatory activity of acidic polysaccharides obtained from the fungus Fomitiporia chilensis. The acidic polysaccharides were obtained for acidic precipitation with 2% O-N-cetyl pyridinium bromide. Chemical analysis was performed by FT-IR and GC-MS methods. The antioxidant capacity of acidic polysaccharides from F. chilensis was evaluated by the scavenging of free radicals with the ABTS and DPPH as-says. Macrophages proliferation and cytokine production assays were used to determine the immunomodulatory capacity of the polysaccharides. Anti-tumor and cytotoxicity ac-tivity was evaluated with MTT assay in the U-937, HTC-116, and HGF-1 cell lines. The ef-fect of polysaccharides on the cell cycle of the HCT-116 cell line was determined for flow cytometry. Fourier Transform-infrared characterization revealed characteristic absorption peaks for polysaccharides, whereas the GC-MS analysis detected three peak correspond-ing to D-galactose, galacturonic acid and D-glucose. The TNF-α proliferation was in-creased when the cell was treated with 2 mg/mL polysaccharides, whereas the IL-6 con-centration was increased with all polysaccharide concentrations evaluated. The cell cycle analysis of HTC-116 treated with polysaccharides evidenced that the acidic polysaccha-rides from F. chilensis induce an increase in the G0/G1 cell cycle phase, incrementing the apoptotic cell percentage. A proteomic analysis suggested some of the molecular mecha-nisms involved in their antioxidant and cellular detoxifying effects and justified their tra-ditional use in heart diseases.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Casimiro Cardenas  

LAB HEAD: Roberto Abdala Díaz

PROVIDER: PXD048361 | Pride | 2024-10-17

REPOSITORIES: Pride