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Proteomics analysis of engineered Paenibacillus polymyxa producing isobutanol

ABSTRACT: This project investigated the protein expression pattern in P. polymyxa at different growth stages. The proteomics provided insights into responses of P. polymyxa to isobutanol and revealed a significant metabolic shift caused by alcohol production. These findings were used to evaluate the bottlenecks and further optimize the pathways towards improving product titers.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Paenibacillus Polymyxa

SUBMITTER: Meliawati Meliawati

LAB HEAD: Jochen Schmid

PROVIDER: PXD048679 | Pride | 2024-05-21

REPOSITORIES: Pride

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			Action	DRS
	20221111_MK_E1200_MVL_FAIMS_1CV_15cm_70min_HRMS1_DIA_1569_prep0010_500ng_1.raw	Raw
	20221111_MK_E1200_MVL_FAIMS_1CV_15cm_70min_HRMS1_DIA_1569_prep0010_500ng_10.raw	Raw
	20221111_MK_E1200_MVL_FAIMS_1CV_15cm_70min_HRMS1_DIA_1569_prep0010_500ng_11.raw	Raw
	20221111_MK_E1200_MVL_FAIMS_1CV_15cm_70min_HRMS1_DIA_1569_prep0010_500ng_12.raw	Raw
	20221111_MK_E1200_MVL_FAIMS_1CV_15cm_70min_HRMS1_DIA_1569_prep0010_500ng_13.raw	Raw

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Engineering the carbon and redox metabolism of Paenibacillus polymyxa for efficient isobutanol production.

Meliawati Meliawati M Volke Daniel C DC Nikel Pablo I PI Schmid Jochen J

Microbial biotechnology 20240301 3

Paenibacillus polymyxa is a non-pathogenic, Gram-positive bacterium endowed with a rich and versatile metabolism. However interesting, this bacterium has been seldom used for bioproduction thus far. In this study, we engineered P. polymyxa for isobutanol production, a relevant bulk chemical and next-generation biofuel. A CRISPR-Cas9-based genome editing tool facilitated the chromosomal integration of a synthetic operon to establish isobutanol production. The 2,3-butanediol biosynthesis pathway, ...[more]

PMID: 38529712

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