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Evaluation of PAC and FASP performance

ABSTRACT: The objective of the experiment was to compare the performance of PAC and FASP.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human) Pisum Sativum (garden Pea) Glycine Max

SUBMITTER: Licia Elvira Prestagiacomo

LAB HEAD: Marco Gaspari

PROVIDER: PXD044349 | Pride | 2024-06-16

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	DIA-NN-FASP-10ug.txt	Txt
	DIA-NN-FASP-1ug.txt	Txt
	DIA-NN-PAC-10ug.txt	Txt
	DIA-NN-PAC-1ug.txt	Txt
	DIA-NN-PAC-SCX-10ug.txt	Txt

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Publications

Evaluation of PAC and FASP Performance: DIA-Based Quantitative Proteomic Analysis.

Murfuni Maria Stella MS Prestagiacomo Licia E LE Giuliano Annarita A Gabriele Caterina C Signoretti Sara S Cuda Giovanni G Gaspari Marco M

International journal of molecular sciences 20240509 10

The aim of this study was to compare filter-aided sample preparation (FASP) and protein aggregation capture (PAC) starting from a three-species protein mix (Human, Soybean and Pisum sativum) and two different starting amounts (1 and 10 µg). Peptide mixtures were analyzed by data-independent acquisition (DIA) and raw files were processed by three commonly used software: Spectronaut, MaxDIA and DIA-NN. Overall, the highest number of proteins (mean value of 5491) were identifie ...[more]

PMID: 38791181

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