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Spatial proteomics mouse intestine

ABSTRACT: Alcohol induced damages to teh crypts and villi of mouse small intestine revealed by laser capture microdissection (LCM)-based sample collection, DIA-based untargeted proteomics

INSTRUMENT(S): Orbitrap Exploris 240

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Ileum

SUBMITTER: Qibin Zhang

LAB HEAD: Qibin Zhang

PROVIDER: PXD048687 | Pride | 2024-05-21

REPOSITORIES: Pride

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			Action	DRS
	Alc_Intestine_LCM_AF_Crypt_1.raw	Raw
	Alc_Intestine_LCM_AF_Crypt_2.raw	Raw
	Alc_Intestine_LCM_AF_Crypt_3.raw	Raw
	Alc_Intestine_LCM_AF_Crypt_4.raw	Raw
	Alc_Intestine_LCM_AF_Crypt_5.raw	Raw

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Publications

Spatial Proteomics Reveals Alcohol-Induced Damages to the Crypts and Villi of the Mouse Small Intestine.

Suresh Patil Shivprasad PS Sun Xinguo X Zhou Zhanxiang Z Zhang Qibin Q

Journal of proteome research 20240424 5

Alcohol consumption perturbs the gut immune barrier and ultimately results in alcoholic liver diseases, but little is known about how immune-related cells in the gut are perturbed in this process. In this study, we employed laser capture microdissection and a label-free proteomics approach to investigate the consequences of alcohol exposure to the proteomes of crypts and villi in the proximal small intestine. Intestinal tissues from alcohol-fed and pair-fed mice were microdissected to selectivel ...[more]

PMID: 38655769

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