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Yeast Serine palmitoyl transferase interaction proteomics

ABSTRACT: ALFA pulldowns of the Lcb1 subunit of the yeast serine palmitoyltransferase. Wt Lcb1 is compared to a mutant lacking the trans membrane helix 1 of Lcb1 and to control pulldowns lackingthe ALFA tag on Lcb1.

INSTRUMENT(S): Bruker Daltonics timsTOF series

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) Saccharomyces Cerevisiae

SUBMITTER: Florian Fröhlich

LAB HEAD: Florian Fröhlich

PROVIDER: PXD048754 | Pride | 2024-09-12

REPOSITORIES: Pride

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The structure of the Orm2-containing serine palmitoyltransferase complex reveals distinct inhibitory potentials of yeast Orm proteins.

Körner Carolin C Schäfer Jan-Hannes JH Esch Bianca M BM Parey Kristian K Walter Stefan S Teis David D Januliene Dovile D Schmidt Oliver O Moeller Arne A Fröhlich Florian F

Cell reports 20240820 8

Sphingolipid levels are crucial determinants of neurodegenerative disorders and therefore require tight regulation. The Orm protein family and ceramides inhibit the rate-limiting step of sphingolipid biosynthesis-the condensation of L-serine and palmitoyl-coenzyme A (CoA). The yeast isoforms Orm1 and Orm2 form a complex with the serine palmitoyltransferase (SPT). While Orm1 and Orm2 have highly similar sequences, they are differentially regulated, though the mechanistic details remain elusive. H ...[more]

PMID: 39167489

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