Proteomics

Dataset Information

0

Yeast Serine palmitoyl transferase interaction proteomics


ABSTRACT: ALFA pulldowns of the Lcb1 subunit of the yeast serine palmitoyltransferase. Wt Lcb1 is compared to a mutant lacking the trans membrane helix 1 of Lcb1 and to control pulldowns lackingthe ALFA tag on Lcb1.

INSTRUMENT(S): Bruker Daltonics timsTOF series

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) Saccharomyces Cerevisiae

SUBMITTER: Florian Fröhlich  

LAB HEAD: Florian Fröhlich

PROVIDER: PXD048754 | Pride | 2024-09-12

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
CRTL_1_1295.d.zip Other
CRTL_2_1296.d.zip Other
CRTL_3_1297.d.zip Other
combined_zip.zip Other
lcb1DTM_1_1287.d.zip Other
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Publications

The structure of the Orm2-containing serine palmitoyltransferase complex reveals distinct inhibitory potentials of yeast Orm proteins.

Körner Carolin C   Schäfer Jan-Hannes JH   Esch Bianca M BM   Parey Kristian K   Walter Stefan S   Teis David D   Januliene Dovile D   Schmidt Oliver O   Moeller Arne A   Fröhlich Florian F  

Cell reports 20240820 8


Sphingolipid levels are crucial determinants of neurodegenerative disorders and therefore require tight regulation. The Orm protein family and ceramides inhibit the rate-limiting step of sphingolipid biosynthesis-the condensation of L-serine and palmitoyl-coenzyme A (CoA). The yeast isoforms Orm1 and Orm2 form a complex with the serine palmitoyltransferase (SPT). While Orm1 and Orm2 have highly similar sequences, they are differentially regulated, though the mechanistic details remain elusive. H  ...[more]

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