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Yeast Serine palmitoyl transferase interaction proteomics

ABSTRACT: ALFA pulldowns of the Lcb1 subunit of the yeast serine palmitoyltransferase. Wt Lcb1 is compared to a mutant lacking the trans membrane helix 1 of Lcb1 and to control pulldowns lackingthe ALFA tag on Lcb1.

INSTRUMENT(S): Bruker Daltonics timsTOF series

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) Saccharomyces Cerevisiae

SUBMITTER: Florian Fröhlich

LAB HEAD: Florian Fröhlich

PROVIDER: PXD048754 | Pride | 2024-09-12

REPOSITORIES: Pride

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			Action	DRS
	CRTL_1_1295.d.zip	Other
	CRTL_2_1296.d.zip	Other
	CRTL_3_1297.d.zip	Other
	combined_zip.zip	Other
	lcb1DTM_1_1287.d.zip	Other

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Publications

The structure of the Orm2-containing serine palmitoyltransferase complex reveals distinct inhibitory potentials of yeast Orm proteins.

Körner Carolin C Schäfer Jan-Hannes JH Esch Bianca M BM Parey Kristian K Walter Stefan S Teis David D Januliene Dovile D Schmidt Oliver O Moeller Arne A Fröhlich Florian F

Cell reports 20240820 8

Sphingolipid levels are crucial determinants of neurodegenerative disorders and therefore require tight regulation. The Orm protein family and ceramides inhibit the rate-limiting step of sphingolipid biosynthesis-the condensation of L-serine and palmitoyl-coenzyme A (CoA). The yeast isoforms Orm1 and Orm2 form a complex with the serine palmitoyltransferase (SPT). While Orm1 and Orm2 have highly similar sequences, they are differentially regulated, though the mechanistic details remain elusive. H ...[more]

PMID: 39167489

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Interaction proteomics of yeast Sac1-ALFA

Project description:Interaction proteomics of yeast Sac1-ALFA compared to untagged control strains

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Sac1 interaction proteomics of ALFA Sac1 and mutants

Project description:Sphingolipids are structural membrane components that also function in cellular stress responses. The serine palmitoyl-transferase (SPT) catalyzes the rate limiting step in sphingolipid biogenesis. Its activity is tightly regulated through multiple bind-ing partners, including Tsc3, Orm proteins, ceramides, and the phosphatidylinositol-4-phosphate (PI4P) phosphatase Sac1. The structural organization and regulatory mechanisms of this complex are not yet understood. Here, we report the high-resolution cryo-EM structures of the yeast SPT in complex with Tsc3 and Orm1 (SPOT) as dimers and monomers and a monomeric complex further carrying Sac1 (SPOTS). In all complexes, the tight interaction of the down-stream metabolite ceramide and Orm1 reveals the ceramide dependent inhibition. Additionally, observation of ceramide and ergosterol binding suggests a co-regulation of sphingolipid biogenesis and sterol metabolism within the SPOTS com-plex.

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Svf1-GFP pulldowns from Limar et al. 2023 JCB

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Identification of distinct active pools of yeast serine palmitoyltransferase in sub-compartments of the ER.

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Evaluation of peptide pulldown coupled to mass spectrometry approaches for investigation of motif-based protein-protein interactions

Project description:Eight different, SLiM-containing peptides were tested for pulldowns immobilized on magnetic beads, and the same sequences were used for PRISMA experiments. Double repeat of peptides were also used for higher local concentration of the motifs.

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Prohibitin cross-linked aided interactome in yeast

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A compendium of RNA-binding motifs for analysis of gene regulation

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Mechanisms of stop codon readthrough mitigation reveal principles of GCN1 mediated translational quality control

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Heterochromatin-associated interactions of Drosophila HP1 with dADD1, HIPP1, and repetitive RNAs

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