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Reversible S-Nitrosylation of bZIP67-Prx IIE Complex by Nitric Oxide Modulates Plant Lipid Content

ABSTRACT: Nitric oxide (NO) is a key gasotransmitter that participates in a broad range of mechanisms controlling plant development and stress conditions. However, little is known about the specific role of this signaling molecule during lipid storage in the seeds. Here, we show NO is accumulated in developing embryos and regulates the fatty acid profile through the stabilization of the basic/leucine zipper transcription factor bZIP67. bZIP67 is a target of NO and nitro-linolenic acid that accumulates to induce the expression of the FAD3 desaturase, which is misregulated in a non-nitrosylable version of the protein. The post-translational modification of bZIP67 is reversed by trans-denitrosylation activity of Peroxiredoxin IIE, defining a feedback mechanism for bZIP redox regulation. These findings led us to provide a molecular framework for the control of seed fatty acid profile cuased by NO, and evidence regarding the in vivo functionality of nitro-fatty acids in plant developmental signaling. To identify new bZIP67 interactors, we performed an in vivo GFP pull-down assay using developing siliques of pbZIP67:GFP-bZIP67 and wild-type Col-0 as control.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Silique

SUBMITTER: Dominique Eeckhout

LAB HEAD: Bert De Rybel

PROVIDER: PXD048872 | Pride | 2024-03-11

REPOSITORIES: Pride

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			Action	DRS
	E13198_2p_BR264_Control_repeat1.raw	Raw
	E13200_2p_BR265_Control_repeat2.raw	Raw
	E13202_2p_BR266_Control_repeat3.raw	Raw
	E13204_2p_BR267_pbzip67-GFP-bzip67_repeat1.raw	Raw
	E13206_2p_BR268_pbzip67-GFP-bzip67_repeat2.raw	Raw

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