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A multi-omics approach to elucidate okadaic acid-induced changes in human HepaRG hepatocarcinoma cells

ABSTRACT: Okadaic acid (OA) is a marine biotoxin that can induce diarrheic shellfish poisoning upon consumption. It can accumulate in filter-feeding shellfish, thereby reaching the human food chain. In addition to inducing severe gastrointestinal symptoms, OA can pass the intestinal barrier into the bloodstream, thereby reaching the liver. However, the effects of OA on the liver have not been much in the focus of research so far. Therefore, this study now focuses on elucidating changes in the proteome of HepaRG cells induced by OA. Furthermore, as a potent phosphatase inhibitor, we expected changes in the phosphoproteome of the cells. Therefore, we further investigated the phosphoproteome of the HepaRG cells after OA exposure. Results revealed changes in enzyme activity regulation, cytoskeleton organization and energy metabolism.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte, Cell Culture

SUBMITTER: Leonie Würger

LAB HEAD: Albert Braeuning

PROVIDER: PXD048968 | Pride | 2024-06-06

REPOSITORIES: Pride

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			Action	DRS
	20230509_HepaRG_LMK24__Phph_enrich_incl_150ng_V2.raw	Raw
	20230509_HepaRG_LMK_05_Phph_enrich_incl_150ng_V2.raw	Raw
	20230509_HepaRG_LMK_4_Phph_enrich_incl_150ng_V2.raw	Raw
	20230509_HepaRG_OA100_05_Phph_enrich_incl_150ng_V2.raw	Raw
	20230509_HepaRG_OA100_24_Phph_enrich_incl_150ng_V2.raw	Raw

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A multi-omics approach to elucidate okadaic acid-induced changes in human HepaRG hepatocarcinoma cells.

Wuerger Leonie T D LTD Sprenger Heike H Krasikova Ksenia K Templin Markus M Stahl Aaron A Herfurth Uta M UM Sieg Holger H Braeuning Albert A

Archives of toxicology 20240604 9

Okadaic acid (OA), a prevalent marine biotoxin found in shellfish, is known for causing acute gastrointestinal symptoms. Despite its potential to reach the bloodstream and the liver, the hepatic effects of OA are not well understood, highlighting a significant research gap. This study aims to comprehensively elucidate the impact of OA on the liver by examining the transcriptome, proteome, and phosphoproteome alterations in human HepaRG liver cells exposed to non-cytotoxic OA concentrations. We e ...[more]

PMID: 38832940

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