Proteomics

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Quantitative LC-MSMS of new hepatoma HepaRG-derived cells obtained using compression forces

ABSTRACT: HepaRG progenitor cells are liver carcinoma cells that exhibit transdifferentiation properties, however, the underlying mechanisms remain poorly known. To evaluate whether a compression stress that mimics the tumoral microenvironment can drive the transdifferentiation of HepaRG cells, we analysed their proteome after modulating the density at which they are grown (compression at high density) and their hormonal environment. We determined a molecular signature of the new HepaRG-derived cell lines, reflecting their differentiation status and stress- and chromatin-related responses. In addition to be a promising way of creating a variety of new cell lines for the study of, e.g., tumoral progression, physical constraint paves the way for the development of new solutions to improve the prognosis of cancer.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Liver Cancer Cell, Regulation Of Hepatocyte Differentiation

SUBMITTER: Fabrice BERTILE  

LAB HEAD: Sarah Cianférani

PROVIDER: PXD036480 | Pride | 2024-05-23

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
MQ_results_HEPARG-derived_CELLS.zip Other
TP1874CMO_Slot2-24_1_1935.d.zip Other
TP1884CMO_Slot2-35_1_1945.d.zip Other
TP1887CMO_Slot2-37_1_1948.d.zip Other
TP1890CMO_Slot2-33_1_1951.d.zip Other
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Publications

Extended lifespan and improved genome stability in HepaRG-derived cell lines through reprogramming by high-density stress.

Brun Charlotte C   Allain Coralie C   Ferron Pierre-Jean PJ   Younoussa Haifaou H   Colicchio Bruno B   Jeandidier Eric E   M'Kacher Radhia R   Guguen-Guillouzo Christiane C   Bertile Fabrice F  

Proceedings of the National Academy of Sciences of the United States of America 20230828 36

The characteristics and fate of cancer cells partly depend on their environmental stiffness, i.e., the local mechanical cues they face. HepaRG progenitors are liver carcinoma cells exhibiting transdifferentiation properties; however, the underlying mechanisms remain unknown. To evaluate the impact of external physical forces mimicking the tumor microenvironment, we seeded them at very high density for 20 h, keeping the cells round and unanchored to the substrate. Applied without corticoids, spat  ...[more]

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