Proteomics

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Widespread prevalence of a post-translational modification in activation of an essential bacterial DNA damage response


ABSTRACT: DNA methylation plays central roles in diverse cellular processes, ranging from error-correction during replication to regulation of bacterial defense mechanisms. Nevertheless, certain aberrant methylation modifications can have lethal consequences. The mechanisms by which bacteria detect and respond to such damage remain incompletely understood. Here, we discover a highly conserved but previously uncharacterized transcription factor (Cada2), which orchestrates a methylation-dependent adaptive response in Caulobacter. This response operates independently of the SOS response, governs the expression of genes crucial for direct repair, and is essential for surviving methylation-induced damage. Our molecular investigation of Cada2 reveals a cysteine methylation-dependent post-translational modification and mode of action distinct from its E. coli counterpart, a trait conserved across all bacteria harboring a Cada2-like homolog instead. Extending across the bacterial kingdom, our findings support the notion of divergence and co-evolution of adaptive response transcription factors and their corresponding sequence-specific DNA motifs. Despite this diversity, the ubiquitous prevalence of adaptive response regulators underscores the significance of a transcriptional switch, mediated by methylation post-translational modification, in driving a specific and essential bacterial DNA damage response.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Caulobacter Crescentus (strain Na1000 / Cb15n)

SUBMITTER: Aditya Kamat  

LAB HEAD: Dr. Anjana Badrinarayanan

PROVIDER: PXD049136 | Pride | 2024-02-09

REPOSITORIES: Pride

Dataset's files

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Action DRS
R114A_xyl_MMS_rep_1.html Other
R114A_xyl_MMS_rep_1.raw Raw
R114A_xyl_MMS_rep_2.html Other
R114A_xyl_MMS_rep_2.raw Raw
R68A_xyl_MMS_rep_1.html Other
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