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COLD6-OSM1 module senses chilling for the tolerance via 2',3'-cAMP signal in rice

ABSTRACT: To further elucidate how COLD6 regulates rice chilling tolerance, we investigated COLD6 interacting proteins using COLD6ind-GFP transgenic line by immunoprecipitation in combination with mass spectrometry (IP-MS). COLD6ind-GFP was applied to bind proteins in chilling-treated rice seedling samples, and the proteins bound at the plasma membrane were subsequently extracted for further analysis. We identified 44 potential interacted proteins with COLD6. Osmotin-like protein (OSM1, LOC_Os12g38170), known for its involvement in plant abiotic stress, was found in two independent experiments.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Plant Cell, Whole Body

SUBMITTER: 伟罗

LAB HEAD: Wei Luo

PROVIDER: PXD049238 | Pride | 2024-11-02

REPOSITORIES: Pride

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			Action	DRS
	IPMSCOLD6GFPrepeat1.group	Other
	IPMSCOLD6GFPrepeat1.mgf	Mgf
	IPMSCOLD6GFPrepeat1.wiff	Wiff
	IPMSCOLD6GFPrepeat1.wiff.scan	Wiff
	IPMSCOLD6GFPrepeat1.xlsx	Xlsx

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Publications

COLD6-OSM1 module senses chilling for cold tolerance via 2',3'-cAMP signaling in rice.

Luo Wei W Xu Yunyuan Y Cao Jie J Guo Xiaoyu X Han Jingdan J Zhang Yuanyuan Y Niu Yuda Y Zhang Meiling M Wang Yi Y Liang Guohua G Qian Qian Q Ge Song S Chong Kang K

Molecular cell 20241030 21

While it is known that temperature sensors trigger calcium (Ca<sup>2+</sup>) signaling to confer cold tolerance in cells, less is known about sensors that couple with other secondary messengers. Here, we identify a cold sensor complex of CHILLING-TOLERANCE DIVERGENCE 6 (COLD6) and osmotin-like 1 (OSM1), which triggers 2',3'-cyclic adenosine monophosphate (2',3'-cAMP) production to enhance cold tolerance in rice. COLD6, which is encoded by a major quantitative trait locus (QTL) gene, interacts wi ...[more]

PMID: 39481382

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