Trypanosoma brucei whole cell proteome changes upon exposure to the aminomethyl-benzoxaborole AN3057
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ABSTRACT: Benzoxaboroles (BoBs) feature a boron-heterocyclic core and are an important recent innovation in the development of drugs against a range of pathogens and other pathologies. A broad spectrum of pharmacology is associated with chemically diverse BoB derivatives and includes multiple modes-of-action and targets. However, a consensus MoA for BoBs targeting evolutionarily diverse protozoan pathogens has emerged with the identification of CPSF3/CPSF73 in the CPSF complex in both apicomplexan and kinetoplastida parasites. We have detected a functional connection between protein sumoylation and the BoB boron-heterocyclic scaffold using comprehensive genetic screens in Trypanosoma brucei. Strikingly, as part of this sumoylation response, members of the CPSF complex are specifically and rapidly destabilised in a SUMO and proteosome-dependent manner. Here we deposit SILAC proteomics data quantifying the effects of BoB exposure on the global protein landscape in T. brucei.
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Trypanosoma Brucei
TISSUE(S): Permanent Cell Line Cell
DISEASE(S): Trypanosomiasis
SUBMITTER: Martin Zoltner
LAB HEAD: Dr. Martin Zoltner
PROVIDER: PXD049328 | Pride | 2024-03-25
REPOSITORIES: Pride
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