Dataset Information

Unraveling the Molecular Mechanism of Biofilm Formation by Rhodanobacter under Environmental Stressors

ABSTRACT: Bacteria are known to adhere to surfaces via self-produced extracellular polymeric substances organized as biofilms. In subsurface areas with low oxygen, limited nutrients, and toxic contaminants, biofilms are crucial for microbial survival and persistence. However, the relationship between biofilm formation and survival in such environments is not well-documented. At the Oak Ridge Reservation Field Research Center (ORRFRC), we observed a high abundance of Rhodanobacter species in conditions with elevated nitrate, metals, organics, and nitric acid. This study investigated the role of biofilm formation in their survival and the underlying molecular mechanisms in diverse geochemical niches. We examined sixteen phylogenetically diverse Rhodanobacter strains for biofilm formation under varying nutrient, pH, and nitrate conditions. Our findings indicate that biofilm formation is a strain-specific phenotype, correlating with environmental stresses, especially in low pH and nitrate conditions. Comparative genomic analysis revealed unique traits in the high biofilm-forming FW021-MT20 strain, such as the absence of flagella and chemotaxis genes and the presence of unique secretion system VI genes, as supported by pangenomic results. Additional tests on biofilm formation in response to field-relevant metals highlighted increased biofilm formation under aluminum stress in strains typically exhibiting weaker biofilm capabilities. Further investigation using RB-Tnseq, proteomics, and TEM indicated flagellar loss under aluminum stress, linked to increased cyclic AMP and di-GMP levels. Our results shed light on the adaptive strategies of Rhodanobacter strains in subsurface environments, suggesting genetic factors linked to biofilm formation and metal stress tolerance, thereby enhancing our understanding of microbial survival under environmental stress.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Rhodanobacter Sp.

SUBMITTER: Christopher Petzold

LAB HEAD: Christopher Petzold

PROVIDER: PXD049428 | Pride | 2025-03-08

REPOSITORIES: pride

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Dataset's files

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			Action	DRS
	10B01_Biofilm_NLDM_AI-R1.raw	Raw
	10B01_Biofilm_NLDM_AI-R2.raw	Raw
	10B01_Biofilm_NLDM_Mn-R1.raw	Raw
	10B01_Biofilm_NLDM_Mn-R2.raw	Raw
	10B01_Biofilm_NLDM_pH4-R1.raw	Raw

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Publications

Molecular mechanisms and environmental adaptations of flagellar loss and biofilm growth of Rhodanobacter under environmental stress.

Chen Mingfei M Trotter Valentine V VV Walian Peter J PJ Chen Yan Y Lopez Romario R Lui Lauren M LM Nielsen Torben N TN Malana Ria Gracielle RG Thorgersen Michael P MP Hendrickson Andrew J AJ Carion Héloïse H Deutschbauer Adam M AM Petzold Christopher J CJ Smith Heidi J HJ Arkin Adam P AP Adams Michael W W MWW Fields Matthew W MW Chakraborty Romy R

The ISME journal 20240101 1

Biofilms aid bacterial adhesion to surfaces via direct and indirect mechanisms, and formation of biofilms is considered as an important strategy for adaptation and survival in suboptimal environmental conditions. However, the molecular underpinnings of biofilm formation in subsurface sediment/groundwater ecosystems where microorganisms often experience fluctuations in nutrient input, pH, and nitrate or metal concentrations are underexplored. We examined biofilm formation under different nutrient ...[more]

PMID: 39113613

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