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High-throughput nano-flow proteomics using a dual-column electrospray source

ABSTRACT: With current trends in proteomics, especially regarding clinical and low input (to single cell) samples, it is increasingly important to both maximize the throughput of the analysis and maintain as much sensitivity as possible. The new generation of mass spectrometers (MS) are taking a huge leap in sensitivity, allowing to analyze samples with shorter liquid chromatography (LC) methods while digging as deep in the proteome. However, the throughput can be doubled by implementing a dual column nano-LC-MS configuration. For this purpose, we used a dual-column setup with a two-outlet electrospray source, and compared it to a classic dual-column setup with a single-outlet source.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: An Staes

LAB HEAD: Simon Devos

PROVIDER: PXD050034 | Pride | 2024-04-22

REPOSITORIES: Pride

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			Action	DRS
	B2912128_Ap_25cmihFrit_PRCRT-229_tandem-twoOutlets-HeLa_500ng-2.raw	Raw
	B2912130_Ap_25cmihFrit_PRCRT-229_tandem-twoOutlets-HeLa_500ng-3.raw	Raw
	B2912132_Ap_25cmihFrit_PRCRT-229_tandem-twoOutlets-HeLa_500ng-4.raw	Raw
	B2912134_Ap_25cmihFrit_PRCRT-229_tandem-twoOutlets-HeLa_500ng-5.raw	Raw
	B2912136_Ap_25cmihFrit_PRCRT-229_tandem-twoOutlets-HeLa_500ng-6.raw	Raw

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