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Top-down mass spectrometry of native proteoforms and their complexes: A community study

ABSTRACT: The combination of native electrospray ionisation with top-down fragmentation in mass spectrometry allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and co-factors. While this approach is powerful, both native mass spectrometry and top-down mass spectrometry are not yet well standardised, and only a limited number of laboratories regularly carry out this type of research. To address this challenge, the Consortium for Top-Down Proteomics (CTDP) initiated a study to develop and test protocols for native mass spectrometry combined with top-down fragmentation of proteins and protein complexes across eleven instruments in nine laboratories. The outcomes are summarised in this report to provide robust benchmarks and a valuable entry point for the scientific community.

INSTRUMENT(S): LTQ Orbitrap, solariX, Synapt MS, 6545 Q-TOF LC/MS, Q Exactive

ORGANISM(S): Bos Taurus (bovine) Escherichia Coli Saccharomyces Cerevisiae (baker's Yeast) Halobacterium Salinarum

SUBMITTER: Tanja Habeck

LAB HEAD: Frederik Lermyte

PROVIDER: PXD047341 | Pride | 2024-05-13

REPOSITORIES: Pride

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			Action	DRS
	Cleavage_Resolution_final.xlsx	Xlsx
	Fragmentation_data_final.xlsx	Xlsx
	Lab1.zip	Other
	Lab2.zip	Other
	Lab3.zip	Other

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