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Mouse cortex immunoprecipitation-mass spectrometry

ABSTRACT: To determine the mechanism for how Kctd10 deficiency impacts brain development, co-immunoprecipitation (co-IP) was performed to identify KCTD10-interacting proteins from the E14.5 cerebral cortex using a KCTD10 antibody, followed by mass spectrometric analysis. In total, 143 proteins uniquely precipitated by the KCTD10 antibody were detected.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: 玲袁

LAB HEAD: Ling Yuan

PROVIDER: PXD050135 | Pride | 2024-05-22

REPOSITORIES: Pride

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			Action	DRS
	AD168GI_IgG.raw	Raw
	AD168GI_KCTD10.raw	Raw
	search.rar	Other

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Publications

KCTD10 regulates brain development by destabilizing brain disorder-associated protein KCTD13.

Cheng Jianbo J Wang Zhen Z Tang Manpei M Zhang Wen W Li Guozhong G Tan Senwei S Mu Chenjun C Hu Mengyuan M Zhang Dan D Jia Xiangbin X Wen Yangxuan Y Guo Hui H Xu Dan D Liu Liang L Li Jiada J Xia Kun K Li Faxiang F Duan Ranhui R Xu Zhiheng Z Yuan Ling L

Proceedings of the National Academy of Sciences of the United States of America 20240315 12

KCTD10 belongs to the KCTD (potassiumchannel tetramerization domain) family, many members of which are associated with neuropsychiatric disorders. However, the biological function underlying the association with brain disorders remains to be explored. Here, we reveal that Kctd10 is highly expressed in neuronal progenitors and layer V neurons throughout brain development. <i>Kctd10</i> deficiency triggers abnormal proliferation and differentiation of neuronal progenitors, reduced deep-layer (espe ...[more]

PMID: 38489388

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