Proteomics

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Use of Proteomics for Dietary Reconstruction: a Case Study using Animal Teeth from Ancient Mesopotamia


ABSTRACT: This research examines animal teeth from Early Dynastic (2900-2350 BC) Mesopotamia (Southern Iraq) to assess animal management practices and identify consumption patterns in animal diets. The objective to answer larger questions about food management and environmental resilience in ancient early complex societies in the Near East was achieved by the use of mass spectrometry-based proteomics for dietary reconstruction. Dietary MS, a revolutionary new methodology applying proteomics techniques to archaeological sample sets to reconstruct ancient animal diet. A developed protein extraction technique followed by liquid chromatography tandem mass spectrometry allowed the identification of the specific plant species consumed in order to highlight variable herd management strategies, resource optimization, for each taxon over time. It also provided information on overall health and indications of disease. This is the first study to apply a full suite of analyses to the region and provides the foundations of a necessary long-term view of human interaction within an environment through both time and space.

INSTRUMENT(S): 6550A iFunnel Q-TOF LC/MS

ORGANISM(S): Environmental Samples <mammals>

TISSUE(S): Dentary Tooth

SUBMITTER: Paulos Chumala  

LAB HEAD: George Katselis

PROVIDER: PXD050140 | Pride | 2025-03-24

REPOSITORIES: Pride

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Publications

Use of Proteomics for Dietary Reconstruction: A Case Study Using Animal Teeth from Ancient Mesopotamia.

Tomilin Megan M   Greenfield Tina T   Chumala Paulos P   Katselis George S GS  

Journal of proteome research 20240815 9


This research examines animal teeth from Early Dynastic (2900-2350 BCE) Mesopotamia (Southern Iraq) to assess animal management practices and identify consumption patterns in animal diets. The objective to answer larger questions about food management and environmental resilience in ancient early complex societies in the Near East was achieved by the use of mass spectrometry-based proteomics for dietary reconstruction. Dietary MS, a revolutionary new methodology applying proteomics techniques to  ...[more]

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