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Electron-Activated Dissociation and Collision-Induced Dissociation Glycopeptide Fragmentation for Improved Glycoproteomics

ABSTRACT: Protein glycosylation is a critical PTM for the stability and biological function of many proteins, but full characterisation of site-specific glycosylation of proteins remains analytically challenging. Collision induced dissociation (CID) is the most common fragmentation method used in LC-MS/MS workflows, but loss of labile modifications render CID inappropriate for detailed characterisation of site-specific glycosylation. Electron-based dissociation (ExD) methods provide alternatives that retain intact glycopeptide fragments for unambiguous site localisation, but these methods often underperform CID due to increased reaction times and reduced efficiency. Electron activated dissociation (EAD) is another strategy for glycopeptide fragmentation. Here, we use a ZenoTOF 7600 SCIEX instrument to compare the performance of various fragmentation techniques for the analysis of a complex mixture of mammalian O- and N-glycopeptides.

INSTRUMENT(S): ZenoTOF 7600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Kyle Macauslane

LAB HEAD: Benjamin L Schulz

PROVIDER: PXD050173 | Pride | 2024-07-04

REPOSITORIES: Pride

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	20230303_R2_01.wiff	Wiff
	20230303_R2_01.wiff.scan	Wiff
	20230303_R2_01.wiff_ECD.byrslt	Wiff
	20230303_R2_01.wiff_ECD.xlsx	Xlsx
	20230303_R2_02.wiff	Wiff

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