Proteomics

Dataset Information

0

HDX-MS analysis of PI4Ka trimer and FAM126a (415-521) with calcineurin

ABSTRACT: We used HDX-MS to map novel binding sites of calcineurin on PI4KA and FAM126A. Calcineurin binds to PxIxIT and LxVP motifs on PI4KA and FAM126A indicating a novel regulatory mechanism of Pi4KA by calcineurin

INSTRUMENT(S): impact HD

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: John Burke  

LAB HEAD: Dr. John E. Burke

PROVIDER: PXD050633 | Pride | 2024-09-03

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
01_26_24_PI4k_CoverageMapping-MSMS.d.rar Other
02_28_24_SS14_P-ND.d.rar Other
02_28_24_SS14_PC_10s-1.d.rar Other
02_28_24_SS14_PC_30s-1.d.rar Other
02_28_24_SS14_PC_3s-1.d.rar Other
Items per page:
1 - 5 of 60
altmetric image

Publications

Structure of calcineurin bound to PI4KA reveals dual interface in both PI4KA and FAM126A.

Shaw Alexandria L AL   Suresh Sushant S   Parson Matthew A H MAH   Harris Noah J NJ   Jenkins Meredith L ML   Yip Calvin K CK   Burke John E JE  

Structure (London, England : 1993) 20240822

Phosphatidylinositol 4-kinase alpha (PI4KA) maintains the phosphatidylinositol 4-phosphate (PI4P) and phosphatidylserine pools of the plasma membrane. A key regulator of PI4KA is its association into a complex with TTC7 and FAM126 proteins. This complex can be regulated by the CNAβ1 isoform of the phosphatase calcineurin. We previously identified that CNAβ1 directly binds to FAM126A. Here, we report a cryoelectron microscopic (cryo-EM) structure of a truncated PI4KA complex bound to calcineurin,  ...[more]

Similar Datasets

Proteomics Hydrogen Deuterium Exchange of PI4K(530-560) Peptide with Calcineurin
2024-09-03 | PXD044636 | Pride
Proteomics Defining the molecular mechanism of PI4KA complex regulation by the phosphatase Calcineurin
2024-09-03 | PXD043409 | Pride
Proteomics HDXMS of PI4KIIIa and Calcineurin
2021-09-22 | PXD025900 | Pride
Proteomics ATP-competitive and allosteric inhibitors induce differential conformational changes at the autoinhibitory interface of Akt
2024-08-23 | PXD039028 | Pride
Proteomics Detailing the Structural and Dynamic Interactions Between WDR44 and Rab11
2023-03-11 | PXD035741 | Pride
Proteomics Structure of the phosphoinositide 3-kinase p110-p101 complex reveals molecular mechanism of GPCR activation
2021-09-22 | PXD025209 | Pride
Proteomics HDX-MS Analysis of CeSpartin and CtSpartinL
2023-12-27 | PXD043175 | Pride
Proteomics Characterization of m-lipin using HDX-MS
2021-06-29 | PXD022172 | Pride
Proteomics HDX-MS Analysis of the protein ALT51
2023-02-22 | PXD034850 | Pride
Proteomics HDX-MS analysis of the mechanism of AKT1 inhibitor binding
2023-02-22 | PXD032929 | Pride