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Assessment of seven minimally sampling protocols of archaeological skeletal proteomes

ABSTRACT: Archaeological bone material is increasingly being sampled for various molecular analyses. Minimally invasive sampling protocols should therefore be utilised whenever possible. We compare the proteomic output of 10 Early Neolithic Bos sp. humeri, from two different preservation contexts, of seven sampling protocols, namely retrieving bone chips (with and without demineralisation), acid etching (FASP and chemical neutralisation), eraser, membrane box, and polishing film. We note that minimally invasive sampling methods can extract a substantial amount of protein groups and provide sufficient taxonomic identifications if preservation conditions are favorable. However, if preservation conditions are poorer more invasive sampling techniques should be considered.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Bone Tissue, Bone

SUBMITTER: Jan Dekker

LAB HEAD: Frido Welker

PROVIDER: PXD050661 | Pride | 2024-11-28

REPOSITORIES: Pride

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			Action	DRS
	20230709_GT_EXPL5_EVO1_15K_30SPD_SAMPLE_P0543.raw	Raw
	20230709_GT_EXPL5_EVO1_15K_30SPD_SAMPLE_P0544.raw	Raw
	20230709_GT_EXPL5_EVO1_15K_30SPD_SAMPLE_P0545.raw	Raw
	20230709_GT_EXPL5_EVO1_15K_30SPD_SAMPLE_P0546.raw	Raw
	20230709_GT_EXPL5_EVO1_15K_30SPD_SAMPLE_P0547.raw	Raw

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