Proteomics

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Complexome profiling of subcellular fractions of Plasmodium falciparum gametocytes

ABSTRACT: The aim of the experiment was to get a better understanding of the complexome of Plasmodium falciparum gametocytes and its subcellular distribution. To do so we, whole parasite cells were isolated and subjected to nitrogen cavitation to free cellular content and homogenize the sample. To decrease sample complexity and obtain subcellular fractions, the homogenate was consequently separated through ultracentrifugation using a discontinuous sucrose gradient. The three resulting fractions were separately resolved by high-resolution clear native electrophoresis (hrCNE) followed by mass spectrometry and complexome profiling analysis using a novel analysis tool we developed, the Gaussian interaction profiler (GIP).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Plasmodium Falciparum

TISSUE(S): Gametocyte

SUBMITTER: Joeri van Strien  

LAB HEAD: Martijn Huynen

PROVIDER: PXD050751 | Pride | 2024-10-17

REPOSITORIES: pride

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20220721_hrCN_Pf_Mito_35-45_14-4_01.raw Raw
20220721_hrCN_Pf_Mito_35-45_14-4_02.raw Raw
20220721_hrCN_Pf_Mito_35-45_14-4_03.raw Raw
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Publications

Analysis of Complexome Profiles with the Gaussian Interaction Profiler (GIP) Reveals Novel Protein Complexes in <i>Plasmodium falciparum</i>.

van Strien Joeri J   Evers Felix F   Cabrera-Orefice Alfredo A   Delhez Iris I   Kooij Taco W A TWA   Huynen Martijn A MA  

Journal of proteome research 20240912 10

Complexome profiling is an experimental approach to identify interactions by integrating native separation of protein complexes and quantitative mass spectrometry. In a typical complexome profile, thousands of proteins are detected across typically ≤100 fractions. This relatively low resolution leads to similar abundance profiles between proteins that are not necessarily interaction partners. To address this challenge, we introduce the Gaussian Interaction Profiler (GIP), a Gaussian mixture mode  ...[more]

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