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Skp1 interactome from WT and PHYA-KO Toxoplasma gondii tachyzoites

ABSTRACT: TgSkp1 interactome from Toxoplasma gondii tachyzoites were performed in 3 Biological Replicates, with 3 Technical Replicates each, by comparing Skp1-SF-TAP (FLAG epitope) tagged strain against parental, non-tagged strain in 2 genetic backgrounds (RHDD - WT and PHYaD - mutant background).

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Toxoplasma Gondii Gt1

TISSUE(S): Cell Culture, Fibroblast

DISEASE(S): Toxoplasmosis

SUBMITTER: Christopher West

LAB HEAD: Christopher West

PROVIDER: PXD050988 | Pride | 2024-09-23

REPOSITORIES: pride

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			Action	DRS
	PhyA_EC_M2_8uL_26Nov20_1.raw	Raw
	PhyA_EC_M2_8uL_26Nov20_2.raw	Raw
	PhyA_EC_M2_8uL_26Nov20_3.raw	Raw
	PhyA_IC_M2_10uL_11Dec20_1.raw	Raw
	PhyA_IC_M2_10uL_11Dec20_2.raw	Raw

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Project description:Toxoplasma gondii is a globally distributed obligate intracellular parasite which can cause zoonotic toxoplasmosis with great harms. The average death time of mice that infected with Toxoplasma gondii RH strain tachyzoites recovered from the liquid nitrogen was shortened after multiple generations. It has been reported that the parasite is in a state of static virulence during cryopreservation and the virulence of the protozoan parasite can be enhanced after continuous passages in hosts under laboratory conditions. However, no research has been conducted to elucidate its biological mechanism. Herein, we sequenced the T. gondii transcriptome using RNA-Seq technology and performed de novo assembly to investigated the virulence factors expression changes by comparing gene expression profiles between incipiently recovered and completely resuscitated tachyzoites. Transcriptome analysis identified 1,951 differentially expressed transcripts in infected liver, of which 1,752 were significantly downregulated and 199 upregulated. We identified many differentially expressed proteins and genes, including serine/threonine kinase, calnexin, myosin and microtubule-associated protein which have previously been reported to be either involved in cell adhesion, parasite gliding or participate in cell invasion. The great majority of the virulence factors including microneme proteins, rhoptry proteins and dense granule proteins were upregulated in fully recovered tachyzoites. The enhanced virulence of recovered Toxoplasma gondii RH strain from the liquid nitrogen is associated with the up-regulated expression of MICs, ROPs and GRAs. Our data will facilitate future genomic research and in-depth annotation of Toxoplasma gondii RH strain genomes. This study provides a profile of the candidate genes that are suspected to be involved with virulence enhancement of recovered Toxoplasma gondii RH strain tachyzoites. Many further studies should be carried out to confirm the function of the candidate genes. Moreover, the preliminary identification of genes and pathways exhibiting differential expression in complete resuscitation stage may further our general understanding of virulence enhancement in this parasite.

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Skp1 interactome from WT and PHYA-KO Toxoplasma gondii tachyzoites

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