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Conventional cell fixation improves performance of in situ crosslinking mass spectrometry while preserving cellular ultrastructure

ABSTRACT: We developed an approach to in situ XL-MS which utilizes pre-stabilization with formaldehyde prior to crosslinker introduction. Applications of this method can boost crosslinker signal and allows for membrane-impermeable reagents to be used for studying protein-interaction landscapes in intact human cells.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Non-small Cell Lung Carcinoma

SUBMITTER: Andrew Michael

LAB HEAD: David C. Schriemer

PROVIDER: PXD051075 | Pride | 2024-04-12

REPOSITORIES: Pride

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			Action	DRS
	A549_01Form_PropNHS1_combined_modified_peptide.tsv	Tabular
	A549_01Form_PropNHS2_combined_modified_peptide.tsv	Tabular
	A549_025Form_PropNHS1_combined_modified_peptide.tsv	Tabular
	A549_025Form_PropNHS2_combined_modified_peptide.tsv	Tabular
	A549_05Form_PropNHS1_combined_modified_peptide.tsv	Tabular

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