Proteomic analysis reveals the high specificity and lack of collateral activity of targeted PspCas13b
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ABSTRACT: To gain a global view on the impact of the collateral activity on protein expression levels, mass spectrometry was used for proteomics analysis of HEK 293T cells when PspCas13b was co-expressed with non-targeted (NT) crRNA or targeting (T) crRNA, which targets the BCR-ABL1 mRNA breakpoint. There was no evidence of off-target protein degradation by T crRNA compared to the non-targeting crRNA. The only significantly repressed proteins were the target BCR-ABL1 p190 (81% reduction) and eGFP (89% reduction) that are encoded on the same mRNA expression construct and encoded mRNA. Catalytic dead Cas13 (dCas13) and crRNA only were used as negative control.
INSTRUMENT(S): Orbitrap Exploris 480
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture, Fibroblast
SUBMITTER: Wenxin Hu
LAB HEAD: Mohamed Fareh
PROVIDER: PXD051089 | Pride | 2024-05-20
REPOSITORIES: Pride
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