Proteomics

Dataset Information

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Proteomic analysis reveals the high specificity and lack of collateral activity of targeted PspCas13b


ABSTRACT: To gain a global view on the impact of the collateral activity on protein expression levels, mass spectrometry was used for proteomics analysis of HEK 293T cells when PspCas13b was co-expressed with non-targeted (NT) crRNA or targeting (T) crRNA, which targets the BCR-ABL1 mRNA breakpoint. There was no evidence of off-target protein degradation by T crRNA compared to the non-targeting crRNA. The only significantly repressed proteins were the target BCR-ABL1 p190 (81% reduction) and eGFP (89% reduction) that are encoded on the same mRNA expression construct and encoded mRNA. Catalytic dead Cas13 (dCas13) and crRNA only were used as negative control.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Wenxin Hu  

LAB HEAD: Mohamed Fareh

PROVIDER: PXD051089 | Pride | 2024-05-20

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
220810_Wenxin_HC-4741_1.raw Raw
220810_Wenxin_HC-4741_10.raw Raw
220810_Wenxin_HC-4741_2.raw Raw
220810_Wenxin_HC-4741_3.raw Raw
220810_Wenxin_HC-4741_4.raw Raw
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