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Proteomics of 24 h TCR activated wild-type and Pim1/Pim2 double knockout CD4 and CD8 T cells

ABSTRACT: T cell antigen-receptor (TCR) and cytokine receptor engagement trigger large changes in Serine/Threonine kinase signalling networks to drive T cell activation and differentiation. The role of only few kinase signalling pathways have been studied in detail, and in this context, Pim kinases are an interesting, yet understudied, family of Serine/Threonine kinases, with reported roles in key processes including survival, proliferation, metabolism across a range of cell types. T lymphocytes predominantly express PIM1 and PIM2, which are rapidly induced by TCR, costimulation and cytokine signalling. Using high-resolution mass spectrometry we systematically examine the impact of Pim1/Pim2 double deficiency downstream of initial TCR engagement. We find that Pim kinases are dispensable for TCR-driven T cell activation and have little impact on the total proteome in these cells.

INSTRUMENT(S): LTQ Orbitrap Velos Pro

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): T Cell, Lymph Node

SUBMITTER: Laura Spinelli

LAB HEAD: Professor Doreen

PROVIDER: PXD051198 | Pride | 2024-04-15

REPOSITORIES: Pride

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	ExperimentalDesignTemplate.txt	Txt
	JM1.raw	Raw
	JM10.raw	Raw
	JM11.raw	Raw
	JM12.raw	Raw

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