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TurboID proximity labelling with protein TRIM52: Identification of biotinylated proteins through pull-down and label-free quantification

ABSTRACT: All constructs were expressed in RKO cells (human rectal carcinoma cell line). A fusion construct of TurboID (mutated BirA* from E. Coli) and green fluorescent protein (GFP) or human TRIM52. Cells were stimulated with Epoxomicin (proteasome inhibitor) or left untreated. Biotinylated proteins were extracted from the cell lysate through pull-down and quantified using label-free MS to identify the proteins in close proximity to TRIM52 using GFP as a specificity control.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: WeiQiang Chen

LAB HEAD: Gijs Versteeg

PROVIDER: PXD051272 | Pride | 2025-03-25

REPOSITORIES: Pride

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			Action	DRS
	20210831_E1_LC8_Hacker_Versteeg_MPL_GFP_EPOX_KH04_35p.raw	Raw
	20210831_E1_LC8_Hacker_Versteeg_MPL_GFP_EPOX_KH05_35p.raw	Raw
	20210831_E1_LC8_Hacker_Versteeg_MPL_GFP_EPOX_KH06_35p.raw	Raw
	20210831_E1_LC8_Hacker_Versteeg_MPL_GFP_UT_KH01_35p.raw	Raw
	20210831_E1_LC8_Hacker_Versteeg_MPL_GFP_UT_KH02_35p.raw	Raw

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